NDLI: Sustained Shoot Multiplication and Method for Overcoming In Vitro Browning in Medicinally Important Plant, Piper chaba Hunt

Content Provider	Springer Nature Link
Author	Rani, Dolly Dantu, Prem Kumar
Copyright Year	2014
Abstract	The current study evaluated for the first time plant regeneration in P. chaba. Adventitious shoot regeneration was achieved in Murashige and Skoog (MS) medium supplemented with three concentrations of 6-benzyladenine (BA; 0.25, 0.5 and 1 mg/l) and 40 mg/l ascorbic acid. Maximum number of 18 adventitious shoot buds was formed in 0.5 mg/l BA but it was accompanied by severe callusing. Therefore, for subsequent multiplication, shoots were subcultured in MS with 0.25 mg/l BA to prevent excessive callusing, though the number of shoots per culture was reduced to 12. A major problem with cultures of this plant was the excessive browning of the shoots and medium due to phenolic exudation. To control browning of tissue and medium two anti-browning agents, ascorbic acid (AA, 10, 40 and 100 mg/l) and activated charcoal (AC, 5 and 10 mg/l) were used individually or in combination with the multiplication medium. Univariate analysis (Kruskal–Wallis test) and principal component analysis plot were performed to assess impact of anti-browning agents on reducing browning but still maintaining consistent rates of proliferation (16.5 ± 0.42) indicated that AC (5 mg/l) was significantly better than either AA alone or any of the combinations of AC and AA. Single shoots were rooted in 0.25 mg/l indole butyric acid and successfully acclimatized under net-house conditions.
Starting Page	407
Ending Page	413
Page Count	7
File Format	PDF
ISSN	03698211
Journal	Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number	86
Issue Number	2
e-ISSN	22501746
Language	English
Publisher	Springer India
Publisher Date	2014-11-26
Publisher Place	New Delhi
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Life Sciences Plant Biochemistry Ascorbic acid Nucleic Acid Chemistry Behavioral Sciences In vitro browning Cluster multiplication Piper chaba Activated charcoal
Content Type	Text
Resource Type	Article
Subject	Environmental Science Agricultural and Biological Sciences

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