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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wood, Andrew Bardag Gorce, Fawzia Sota, Hiroyuki Oliva, Joan Niihara, Yutaka |
| Copyright Year | 2015 |
| Abstract | Autologous stem cell transplantation for eye diseases is immunologically preferable to avoid allograft rejection. However, the fate of the grafted cells has never been studied. Here, we propose to use $^{19}$F-perfluorocarbon magnetic resonance imaging tracer agent, to label cell sheet in vitro. This labeling enables non-invasive visualization of possible migration of grafted cells. Oral mucosal epithelial cells were isolated from rabbit oral mucosal epithelium and were cultivated in a thermo-responsive surface to engineer a multilayer cell sheet. Different concentrations of $^{19}$F-perfluorocarbon were added to the cell sheet culture media, one or two times. Cells were analyzed in a 7 T nuclear magnetic resonance to determine the labeling efficiency. We found that 10 mg/mL and two incubations with $^{19}$F-perfluorocarbon were the optimal condition for labeling. H&E and immunocytochemistry showed that labeling did not affect the expression of cell sheets specific markers (CK4, CK13, connexin43, E-cadherin). Furthermore, no significant effects were observed on the number of cells and the cell viability, making $^{19}$F-perfluorocarbon suitable for cell tracking, with no side effects. |
| Starting Page | 371 |
| Ending Page | 378 |
| Page Count | 8 |
| File Format | |
| ISSN | 17382696 |
| Journal | Tissue Engineering and Regenerative Medicine |
| Volume Number | 12 |
| Issue Number | 5 |
| e-ISSN | 22125469 |
| Language | English |
| Publisher | Korean Tissue Engineering and Regenerative Medicine Society |
| Publisher Date | 2015-10-25 |
| Publisher Place | Seoul |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biomedical Engineering Stem Cells Regenerative Medicine/Tissue Engineering $^{19}$F-perfluorocarbon Cell sheet Nuclear magnetic resonance Oral mucosa epithelial Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biomedical Engineering |
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