NDLI: Sequential differentiation of human bone marrow stromal cells for bone regeneration

Content Provider	Springer Nature Link
Author	Kubosch, David Suedkamp, Norbert P. Padron, Nestor Torio Huebner, Eva Johanna Niemeyer, Philipp Finkenzeller, Guenter
Copyright Year	2015
Abstract	In this study we hypothesized that as a simulation of endochondral bone formation, bone marrow stromal cell (BMSC) provide a sequential chondro-osteogenic differentiation potential. A chondrogenic priming of BMSC leads to a spontaneous three-dimensional cell formation. BMSC were chondrogenically differentiated prior to an osteogenic stimulation. Duration of cell culture was 28 days, whereas in group A BMSC were chondrogenically differentiated for 1 day, followed by an osteogenic differentiation for 27 days. In group B BMSC were chondrogenically differentiated for 14 days prior to an osteogenic differentiation of 14 days and group C BMSC were differentiated chondrogenically for 28 days serving as a chondrogenic control group. Chondrogenic priming induced a spontaneous three-dimensional cell formation. To survey the stability of the osteogenic phenotype in the absence of an osteogenic stimulus, investigations were performed in vivo in a specially adapted chorioallantoic membrane model of fertilized White Leghorn eggs. Histology and real time polymerase chain reaction revealed a higher amount of osteogenic extracellular matrix synthesis and significant higher expressions of osteogenic marker genes in group B after 14 days of chondrogenic and 14 days of osteogenic stimulation. Matrix calcification in vivo in the absence of an osteogenic stimulus could be demonstrated. The results of the present study support the theory of a sequential differentiation potential of BMSC. A chondrogenic priming of BMSC stimulated into the osteogenic lineage result in a stable osteogenic phenotype in a scaffold-free, three-dimensional tissue engineering application.
Starting Page	331
Ending Page	342
Page Count	12
File Format	PDF
ISSN	17382696
Journal	Tissue Engineering and Regenerative Medicine
Volume Number	12
Issue Number	5
e-ISSN	22125469
Language	English
Publisher	Korean Tissue Engineering and Regenerative Medicine Society
Publisher Date	2015-10-25
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomedical Engineering Stem Cells Tissue engineering Bone regeneration Pellet culture Regenerative Medicine/Tissue Engineering Bone marrow stromal cells Bone defect Cell Biology
Content Type	Text
Resource Type	Article
Subject	Medicine Biomedical Engineering

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