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  1. Journal of the Korean Society for Applied Biological Chemistry
  2. Journal of the Korean Society for Applied Biological Chemistry : Volume 52
  3. Journal of the Korean Society for Applied Biological Chemistry : Volume 52, Issue 4, August 2009
  4. Cloning and characterization of the UDP glucose/galactose epimerases of Oryza sativa
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Journal of the Korean Society for Applied Biological Chemistry : Volume 60
Journal of the Korean Society for Applied Biological Chemistry : Volume 59
Journal of the Korean Society for Applied Biological Chemistry : Volume 58
Journal of the Korean Society for Applied Biological Chemistry : Volume 57
Journal of the Korean Society for Applied Biological Chemistry : Volume 56
Journal of the Korean Society for Applied Biological Chemistry : Volume 55
Journal of the Korean Society for Applied Biological Chemistry : Volume 54
Journal of the Korean Society for Applied Biological Chemistry : Volume 53
Journal of the Korean Society for Applied Biological Chemistry : Volume 52
Journal of the Korean Society for Applied Biological Chemistry : Volume 52, Issue 6, December 2009
Journal of the Korean Society for Applied Biological Chemistry : Volume 52, Issue 5, October 2009
Journal of the Korean Society for Applied Biological Chemistry : Volume 52, Issue 4, August 2009
Characterization of facultative sulfur-oxidizing Marinobacter sp. BR13 isolated from marine sediment of Yellow Sea, Korea
Cloning and characterization of the UDP glucose/galactose epimerases of Oryza sativa
Flavonoids as potential inhibitors of retroviral enzymes
Antimicrobial effects of anthracene and its derivatives against intestinal bacteria
Growth inhibiting activity of quinaldic acid isolated from Ephedra pachyclada against intestinal bacteria
Isolation and identification of lipids from the silkworm (Bombyx mori) droppings
Inhibitory effects of stilbene glucoside isolated from the root of Polygonum multiflorum on tyrosinase activity and melanin biosynthesis
Metabolic profiling and discrimination of two cacti cultivated in Korea using HPLC-ESI-MS and multivariate statistical analysis
Comparative repellency of essential oils against Culex pipiens pallens (Diptera: Culicidae)
Antioxidant and anti-inflammatory activity of Kalopanax pictus leaf
Anti-Helicobacter pylori diarylheptanoid identified in the rhizome of Alpinia officinarum
Inhibitory effects of 2-(4-chlorophenyl)-1,3-selenazol-4-one on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells
Antitumor components isolated from the heartwood extract of Dalbergia odorifera
An enhanced ELISA for the acaricide/insecticide fenazaquin
Effect of inorganic and organic germanium treatments on the growth of lettuce (Lactuca sativa)
Reduction of shoot hyperhydricity in micropropagated potato plants via antisense inhibition of a chCu/ZnSOD gene
Accumulation of intracellular ATP is a factor suppressing morphological differentiation in adenosine kinase mutant of Streptomyces lividans
Journal of the Korean Society for Applied Biological Chemistry : Volume 52, Issue 3, June 2009
Journal of the Korean Society for Applied Biological Chemistry : Volume 52, Issue 2, April 2009
Journal of the Korean Society for Applied Biological Chemistry : Volume 52, Issue 1, February 2009

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Cloning and characterization of the UDP glucose/galactose epimerases of Oryza sativa

Content Provider Springer Nature Link
Author Kim, Bong Gyu Hong, Byoung Seok Ahn, Joong Hoon Jeon, Young Min Kim, Seong Kyong Kim, Dae Hwan
Copyright Year 2003
Abstract UDP-glucose/galactose-4-epimerase (UGE) catalyzes the interconversion of UDP-glucose and UDP-galactose. Oryza sativa contains four UGEs. Expression of OsUGE3 was higher than other OsUGEs in all tissues except the stem. OsUGE1 expression was enhanced after drought, salt or UV-irradiation stress. Four rice UGEs (OsUGE1–4) were cloned by polymerase chain reaction and sequenced. OsUGE1–4 were expressed in Escherichia coli with glutathione S-transferase fusion protein and purified. All four OsUGEs could covert UDP-glucose into UDP-galactose better than vice versa. Kinetic parameters of OsUGEs showed that OsUGE1 was most efficient. These OsUGEs could be used for biosynthesis of various UDP-sugars, which serve as a cosubstrates of UDP-dependent glycosyltransferase modification reactions of antibiotics or flavonoids.
Starting Page 315
Ending Page 320
Page Count 6
File Format PDF
ISSN 17382203
Journal Journal of the Korean Society for Applied Biological Chemistry
Volume Number 52
Issue Number 4
e-ISSN 2234344X
Language English
Publisher Springer-Verlag
Publisher Date 2003-01-01
Publisher Place New York
Access Restriction Subscribed
Subject Keyword Oryza sativa Applied Microbiology UDP-glucose/galactose-4-epimerase Biological Techniques UDP-sugar Bioorganic Chemistry
Content Type Text
Resource Type Article
Subject Organic Chemistry Biochemistry, Genetics and Molecular Biology
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