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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kim, Bong Gyu Hong, Byoung Seok Ahn, Joong Hoon Jeon, Young Min Kim, Seong Kyong Kim, Dae Hwan |
| Copyright Year | 2003 |
| Abstract | UDP-glucose/galactose-4-epimerase (UGE) catalyzes the interconversion of UDP-glucose and UDP-galactose. Oryza sativa contains four UGEs. Expression of OsUGE3 was higher than other OsUGEs in all tissues except the stem. OsUGE1 expression was enhanced after drought, salt or UV-irradiation stress. Four rice UGEs (OsUGE1–4) were cloned by polymerase chain reaction and sequenced. OsUGE1–4 were expressed in Escherichia coli with glutathione S-transferase fusion protein and purified. All four OsUGEs could covert UDP-glucose into UDP-galactose better than vice versa. Kinetic parameters of OsUGEs showed that OsUGE1 was most efficient. These OsUGEs could be used for biosynthesis of various UDP-sugars, which serve as a cosubstrates of UDP-dependent glycosyltransferase modification reactions of antibiotics or flavonoids. |
| Starting Page | 315 |
| Ending Page | 320 |
| Page Count | 6 |
| File Format | |
| ISSN | 17382203 |
| Journal | Journal of the Korean Society for Applied Biological Chemistry |
| Volume Number | 52 |
| Issue Number | 4 |
| e-ISSN | 2234344X |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2003-01-01 |
| Publisher Place | New York |
| Access Restriction | Subscribed |
| Subject Keyword | Oryza sativa Applied Microbiology UDP-glucose/galactose-4-epimerase Biological Techniques UDP-sugar Bioorganic Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Biochemistry, Genetics and Molecular Biology |
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