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Flavonoids from Fragaria ananassa calyx and their antioxidant capacities

Content Provider	Springer Nature Link
Author	Baek, Nam In Baek, Yoon Su Song, Na Young Kwon, Oh Keun Kim, Dae Ok Nam, Tae Gyu Kang, Hee Cheol
Copyright Year	2015
Abstract	The calyx of Fragaria ananassa (Rosaceae) has not been investigated for its chemical constituents and biological activity. This study focused on the isolation of active metabolites from the calyx of this plant. Compounds from F. ananassa calyx were extracted in 80 % aqueous MeOH and the concentrated extract was successively partitioned into EtOAc, n-BuOH, and H$_{2}$O fractions. Repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies afforded six purified flavonoids. On the basis of spectroscopic methods, including $^{1}$H, $^{13}$C nuclear magnetic resonance spectrometry (NMR), DEPT, and 2D NMR (gCOSY, gHSQC, gHMBC), the flavonoids were identified as kaempferol (1), astragalin (2), quercetin (3), isoquercitrin (4), sexangularetin (5), and sinensin (6). All the flavonoids were isolated for the first time from the F. ananassa calyx in this study. The flavonoids were evaluated for radical-scavenging capacity 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt), 2,2-diphenyl-1-picrylhydrazyl, and oxygen radical absorbance capacity methods. In conclusion, quercetin (3) consistently showed the highest ABTS, DPPH, and ORAC radical-scavenging activities compared with the other isolated flavonoids.
Starting Page	787
Ending Page	793
Page Count	7
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	58
Issue Number	6
e-ISSN	2234344X
Language	English
Publisher	The Korean Society for Applied Biological Chemistry
Publisher Date	2015-07-31
Publisher Place	Seoul
Access Restriction	Subscribed
Subject Keyword	Fragaria ananassa calyx Radical-scavenging capacity Applied Microbiology Biological Techniques 2,2-Diphenyl-1-picrylhydrazyl Oxygen radical absorbance capacity Antioxidant Flavonoid Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

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