NDLI: Tryptophan enhanced accumulation of phenolic compounds via chorismate mutase activation in the Ganoderma neo-japonicum mycelia

Content Provider	Springer Nature Link
Author	Park, Eung Jun Lee, Wi Young
Copyright Year	2003
Abstract	The aim of this study was to investigate the effect of tryptophan (Trp) on the production of total phenolic compounds (TP) in the mycelial culture of Ganoderma neo-japonicum (G. neo-japonicum). Among various amino acids tested, Trp was best for increasing the TP content by 13.2-fold compared to the control. Trp also increased the phenylalanine content via Trp-specific activation of chorismate mutase. Although 12 mM of Trp was the optimal concentration for TP production, it significantly inhibited mycelial growth. This growth inhibition was reversed by the addition of yeast extract (YE) in a dose-dependent manner. Consequently the combined addition of Trp (12 mM) and YE (8 g/L) stimulated TP production up to 73.5 mg gallic acid equivalent per g dry weight of mycelial culture. Furthermore, the phenolic profiling revealed that quercertin occupied 34.1% of the total amount of phenolic compounds tested and the resveratrol concentration increased at the highest rate (49.1-fold) compared to that of the control. These results provided strong evidence that the accumulation of phenolic compounds in the G. neo-japonicum mycelia was regulated by the availability of substrates involved in the phenylpropanoid pathway, which may be useful in regulation and optimization of G. neo-japonicum culture for efficient production of valuable nutraceuticals.
Starting Page	364
Ending Page	370
Page Count	7
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	53
Issue Number	3
e-ISSN	2234344X
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	mycelial culture Ganoderma neo-japonicum phenolic compounds Applied Microbiology Biological Techniques tryptophan Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

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