NDLI: Proteomic Analysis of Seed Storage Proteins in Low Allergenic Soybean Accession

Content Provider	Springer Nature Link
Author	Seo, Jun Su Koo, Sung Cheol Choi, Man Soo Park, Kyoung Mi Kim, Min Chul Bae, Dong Won Kim, Kyeong Moon Kim, Seok Hyeon Shim, Sang In Chung, Jong Il
Copyright Year	2003
Abstract	Soybean (Glycine max L. Merr.) is a major source of protein for human and animal nutrients. However, soybean seed also contains a number of allergenic proteins, which limit its extensive usage in the food industry. P34 (Gly m Bd 30K), a low-leveled but highly conserved seed storage protein, has been described as a major allergen in soybean seed. Recently, two low P34 soybean accessions, PI603570A and PI567476, were identified by extensive screening of soybean germplasm. We have surveyed spatiotemporal expression pattern of P34 protein in low P34 accession, PI567476, and cultivated soybean accession, Clark, using antibody against P34. Immunoblot analysis showed not only seed specific expression pattern of P34, but also significantly reduced expression of P34 in PI567476 compared to Clark. Additionally, we performed proteomic analysis to compare protein compositions between PI567476 and Clark accessions. Although the overall distribution pattern of the protein spots was similar in both soybean accessions, 19 protein spots showing different intensity between both accessions were detected and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). PI567476 accession exhibited altered expression of several seed storage proteins including allergens and seed maturation-related proteins. The detailed analysis for composition of seed storage proteins of the PI567476 accession provides valuable information in use of this soybean germplasm for breeding of low allergenic elite soybean lines, which is expected to enhance food safety and make soy products available to allergen-sensitive individuals.
Starting Page	332
Ending Page	339
Page Count	8
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	54
Issue Number	3
e-ISSN	2234344X
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	P34 soybean allergen germplasm seed storage protein Applied Microbiology Biological Techniques Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Biochemical and proteomic analysis of soybean sprouts at different germination temperatures

Proteomic analysis of differentially expressed proteins of rice in response to cadmium

Application of simple biological analyses to screen irradiated brown rice, soybean and sesame seeds

Immuno-affinity purification of 2B8-tagged proteins

Genetic and molecular regulation of flower pigmentation in soybean

Erratum to: Flavonoids activating adenosine monophosphate-activated protein kinase

Profiling of differentially expressed proteins in mature kernels of Korean waxy corn cultivars using proteomic analysis

Proteomic analysis of hepatotoxicity induced by titanium nanoparticles in mouse liver

Effect of lotus seed and seedpod extracts on oxidative stability against lard during storage

Proteomic Analysis of Seed Storage Proteins in Low Allergenic Soybean Accession

Similar Documents

Biochemical and proteomic analysis of soybean sprouts at different germination temperatures

Proteomic analysis of differentially expressed proteins of rice in response to cadmium

Application of simple biological analyses to screen irradiated brown rice, soybean and sesame seeds

Immuno-affinity purification of 2B8-tagged proteins

Genetic and molecular regulation of flower pigmentation in soybean

Erratum to: Flavonoids activating adenosine monophosphate-activated protein kinase

Profiling of differentially expressed proteins in mature kernels of Korean waxy corn cultivars using proteomic analysis

Proteomic analysis of hepatotoxicity induced by titanium nanoparticles in mouse liver

Effect of lotus seed and seedpod extracts on oxidative stability against lard during storage

Proteomic Analysis of Seed Storage Proteins in Low Allergenic Soybean Accession