NDLI: Nicotine detoxification of rutin, quercitrin, and chlorogenic acid isolated from Houttuynia cordata by reducing reactive oxygen species and inducing conversion from nicotine to cotinine

Content Provider	Springer Nature Link
Author	Kim, Kyeong Mu Shim, Soon Mi
Copyright Year	2014
Abstract	The hypothesis of the present study is that methanol extract of Houttuynia cordata Thunb (MEH) and its targeted bioactive components including rutin, quercitrin, and chlorogenic acid can be effective in reducing reactive oxygen species (ROS) caused by nicotine and promoting nicotine to cotinine in HepG2 cell. Oxygen radical absorbance capacity (ORAC) of bioactive components and MEH was measured to assess free radical scavenging capacity. ROS inhibition ability of bioactive components and MEH was measured by 2′,7′-dichlorodihydrofluorescein diacetate assay. The conversion rate of nicotine to cotinine by bioactive components and MEH was determined by the direct barbiturate assay method. ORAC value confirmed that MEH and its bioactive components provided an antioxidant capacity ranging from 126 to 138 μMtrolox equivalents/100 g. Compared to nicotine only, pretreatment of MEH, rutin, and quercitrin was revealed to effectively inhibit ROS production in HepG2 cell by up to 9, 7.4, and 14%, respectively. Nicotine conversion to cotinine after 120 min incubation was 1.7 and 1.4 times higher in rutin and chlorogenic acid pretreatment than the control, respectively. H. cordata and its targeted bioactive components could be a valuable natural ingredient for inhibiting ROS formation by nicotine as well as enhancing the rate of nicotine to cotinine turnover.
Starting Page	503
Ending Page	509
Page Count	7
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	57
Issue Number	4
e-ISSN	2234344X
Language	English
Publisher	The Korean Society for Applied Biological Chemistry
Publisher Date	2014-08-07
Publisher Place	Seoul
Access Restriction	Subscribed
Subject Keyword	nicotine oxygen radical absorbance capacity Houttuynia cordata Thunb cotinine reactive oxygen species Applied Microbiology Biological Techniques HepG2 cell Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

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