NDLI: Novel functional hepatocyte cell line derived from spontaneous dwarf rat: Model of growth hormone function in vitro

Content Provider	Springer Nature Link
Author	Ishikawa, Mayumi Tachibana, Toshiaki Yoshino, Gen Hashimoto, Hisashi Tanaka, Toshiaki
Copyright Year	2010
Abstract	Currently there is no good hepatocyte model for studying growth hormone (GH) function that reflects its normal physiological roles. Here we report the establishment of a functional hepatocyte cell line, SDRL-1, from the liver of young male spontaneous dwarf rats (SDR), with isolated GH deficiency. This line has been maintained in Dulbecco’s Modified Eagle Medium (DMEM)/F12 medium supplemented with 10% fetal bovine serum (FBS) with retention of a near diploid karyotype for extended periods of time. When grown as a monolayer sheet, it displayed a pavement-like appearance and contact inhibition. These cells have a poorly developed rough endoplasmic reticulum (r-ER), few mitochondria and glycogen granules, and produce a small amount of albumin and α-fetoprotein, that is enhanced when grown on a collagen gel sponge. Human recombinant GH stimulated JAK2 and STAT5b tyrosine phosphorylation and IGF-I production in a concentration-dependent manner. When the cells were cultured with GH-supplemented medium, the number of mitochondria and glycogen granules increased together with the r-ER and Golgi apparatus. A number of microvilli were observed on the surface of the cultured cells, further suggesting that this cell line is composed of normally functioning hepatocytes. In summary, we established a novel hepatocyte cell line (SDRL-1), that appears to display normal function, which we propose can serve as a good in vitro model for studying GH-target organ interactions.
Starting Page	164
Ending Page	172
Page Count	9
File Format	PDF
ISSN	09147470
Journal	Human Cell
Volume Number	23
Issue Number	4
e-ISSN	17490774
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-01-01
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	growth hormone hepatocyte cell line spontaneous dwarf rat Cell Biology Embryology Oncology Stem Cells Reproductive Medicine Cell Culture
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cancer Research

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

New approach for the establishment of an hepatocyte cell line derived from rat early embryonic stem cells

Novel method for the establishment of cardiomyocytes derived from rat embryonic stem cells in vitro

Differentiation of mesenchymal cells derived from human amniotic membranes into hepatocyte-like cells in vitro

Functional analysis of three novel cell lines derived from human papillary thyroid carcinomas with three different clinical courses

Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin

Establishment and characterization of a new human glioblastoma cells line, NYGM

Induced in vitro differentiation of neural-like cells from human amnion-derived fibroblast-like cells

Establishment and characterization of a new squamous cell carcinoma cell line, TMUU-08, derived from human bladder cancer

Establishment and characterization of human malignant lymphoma cell line derived from uterine cervix

Novel functional hepatocyte cell line derived from spontaneous dwarf rat: Model of growth hormone function in vitro

Similar Documents

New approach for the establishment of an hepatocyte cell line derived from rat early embryonic stem cells

Novel method for the establishment of cardiomyocytes derived from rat embryonic stem cells in vitro

Differentiation of mesenchymal cells derived from human amniotic membranes into hepatocyte-like cells in vitro

Functional analysis of three novel cell lines derived from human papillary thyroid carcinomas with three different clinical courses

Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin

Establishment and characterization of a new human glioblastoma cells line, NYGM

Induced in vitro differentiation of neural-like cells from human amnion-derived fibroblast-like cells

Establishment and characterization of a new squamous cell carcinoma cell line, TMUU-08, derived from human bladder cancer

Establishment and characterization of human malignant lymphoma cell line derived from uterine cervix

Novel functional hepatocyte cell line derived from spontaneous dwarf rat: Model of growth hormone function in vitro