NDLI: Different responses between diploid and tetraploid H1 embryonic stem cells appeared during long-term culturing in L15F10 medium without leukemia inhibitory factor

Content Provider	Springer Nature Link
Author	Luo, Xianwen Fujikawa Yamamoto, Kohzaburo Miyagoshi, Minoru Yamagishi, Hiroko
Copyright Year	2010
Abstract	To examine the alteration in cellular characteristics of polyploid embryonic stem (ES) cells during long-term culturing without leukemia inhibitory factor (LIF), mouse diploid and tetraploid H-1 (ES) cells (2H1 and 4H1 cells, respectively) were cultured without LIF for approximately 5 months. 2H1 and 4H1 cells were adapted to the medium without LIF by decreasing the concentration for several passages, and they were denoted as 2H1$^{(−)}$ and 4H1$^{(−)}$ cells, respectively. DNA content of 4H1$^{(−)}$ cells decreased gradually in the early stage, increased abruptly in the second stage, and then was maintained for a long time. 4H1$^{(−)}$ cells exhibited longer doubling time and equivalent phase fraction compared with those of 2H1$^{(−)}$ cells. The G$_{1}$ phase fractions of 2H1$^{(−)}$ and 4H1$^{(−)}$ cells were increased compared with that of 2H1 cells. Cellular morphology and pluripotency were maintained in 4H1$^{(−)}$ cells but not in 2H1$^{(−)}$ cells. 2H1$^{(−)}$ cells showed a cell population consisting of several kinds of cells, and they lost alkaline phosphatase activity, suggesting that the cells had differentiated. 4H1$^{(−)}$ cells, however, exhibited alkaline phosphatase activity and formed teratocarcinoma in mouse abdomen, suggesting that the cells maintained their pluripotency in the medium without LIF.
Starting Page	134
Ending Page	140
Page Count	7
File Format	PDF
ISSN	09147470
Journal	Human Cell
Volume Number	23
Issue Number	4
e-ISSN	17490774
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-01-01
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	H-1 (ES) cells L15F10 medium long-term culturing tetraploid without LIF Cell Biology Embryology Oncology Stem Cells Reproductive Medicine Cell Culture
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cancer Research

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