NDLI: Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals

Please wait, while we are loading the content...

Early detection of neuropathophysiology using diffusion-weighted magnetic resonance imaging in asymptomatic cats with feline immunodeficiency viral infection

The type I interferon response bridles rabies virus infection and reduces pathogenicity

Regional areas and widths of the midsagittal corpus callosum among HIV-infected patients on stable antiretroviral therapies

Erratum to: Regional areas and widths of the midsagittal corpus callosum among HIV-infected patients on stable antiretroviral therapies

Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals

Colorado alphaherpesvirus latency symposium

Journal of NeuroVirology : Volume 17, Issue 3, June 2011

Journal of NeuroVirology : Volume 17, Issue 2, April 2011

Journal of NeuroVirology : Volume 17, Issue 1, February 2011

Journal of NeuroVirology : Volume 16

Journal of NeuroVirology : Volume 15

Journal of NeuroVirology : Volume 14

Journal of NeuroVirology : Volume 13

Journal of NeuroVirology : Volume 12

Journal of NeuroVirology : Volume 11

Journal of NeuroVirology : Volume 10

Journal of NeuroVirology : Volume 9

Journal of NeuroVirology : Volume 8

Journal of NeuroVirology : Volume 7

Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals

Content Provider	Springer Nature Link
Author	Brown, Amanda Islam, Tanzeem Adams, Robert Nerle, Sujata Kamara, Masiray Eger, Caitlin Marder, Karen Cohen, Bruce Schifitto, Giovanni McArthur, Justin C. Sacktor, Ned Pardo, Carlos A.
Copyright Year	2011
Abstract	Despite effective and widely available suppressive anti-HIV therapy, the prevalence of mild neurocognitive dysfunction continues to increase. HIV-associated neurocognitive disorder (HAND) is a multifactorial disease with sustained central nervous system inflammation and immune activation as prominent features. Inflammatory macrophages, HIV-infected and uninfected, play a central role in the development of HIV dementia. There is a critical need to identify biomarkers and to better understand the molecular mechanisms leading to cognitive dysfunction in HAND. In this regard, we identified through a subtractive hybridization strategy osteopontin (OPN, SPP1, gene) an inflammatory marker, as an upregulated gene in HIV-infected primary human monocyte-derived macrophages. Knockdown of OPN in primary macrophages resulted in a threefold decrease in HIV-1 replication. Ectopic expression of OPN in the TZM-bl cell line significantly enhanced HIV infectivity and replication. A significant increase in the degradation of the NF-κB inhibitor, IκBα and an increase in the nuclear-to-cytoplasmic ratio of NF-κB were found in HIV-infected cells expressing OPN compared to controls. Moreover, mutation of the NF-κB binding domain in the HIV-LTR abrogated enhanced promoter activity stimulated by OPN. Interestingly, compared to cerebrospinal fluid from normal and multiple sclerosis controls, OPN levels were significantly higher in HIV-infected individuals both with and without neurocognitive disorder. OPN levels were highest in HIV-infected individuals with moderate to severe cognitive impairment. Moreover, OPN was significantly elevated in brain tissue from HIV-infected individuals with cognitive disorder versus those without impairment. Collectively, these data suggest that OPN stimulates HIV-1 replication and that high levels of OPN are present in the CNS compartment of HIV-infected individuals, reflecting ongoing inflammatory processes at this site despite anti-HIV therapy.
Starting Page	382
Ending Page	392
Page Count	11
File Format	PDF
ISSN	13550284
Journal	Journal of NeuroVirology
Volume Number	17
Issue Number	4
e-ISSN	15382443
Language	English
Publisher	Springer US
Publisher Date	2011-05-10
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	HIV-associated neurocognitive disorder CD44 Nef Infectious Diseases Virology Immunology Neurosciences Neurology
Content Type	Text
Resource Type	Article
Subject	Neurology Virology Neurology (clinical) Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals

Cerebrospinal fluid biomarkers and HIV-associated neurocognitive disorders in HIV-infected individuals in Rakai, Uganda

Neurocognitive impairment in HIV-1 clade C- versus B-infected individuals in Southern Brazil

Increased blood-brain barrier permeability in neuro-asymptomatic HIV-1-infected individuals—correlation with cerebrospinal fluid HIV-1 RNA and neopterin levels

Cerebrospinal fluid cell-free mitochondrial DNA is associated with HIV replication, iron transport, and mild HIV-associated neurocognitive impairment

Cerebrospinal fluid interferon alpha levels correlate with neurocognitive impairment in ambulatory HIV-Infected individuals

Accelerated epigenetic aging in brain is associated with pre-mortem HIV-associated neurocognitive disorders

Chemokines in cerebrospinal fluid correlate with cerebral metabolite patterns in HIV-infected individuals

Biomarkers of chemotaxis and inflammation in cerebrospinal fluid and serum in individuals with HIV-1 subtype C versus B

Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals

Similar Documents

Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals

Cerebrospinal fluid biomarkers and HIV-associated neurocognitive disorders in HIV-infected individuals in Rakai, Uganda

Neurocognitive impairment in HIV-1 clade C- versus B-infected individuals in Southern Brazil

Increased blood-brain barrier permeability in neuro-asymptomatic HIV-1-infected individuals—correlation with cerebrospinal fluid HIV-1 RNA and neopterin levels

Cerebrospinal fluid cell-free mitochondrial DNA is associated with HIV replication, iron transport, and mild HIV-associated neurocognitive impairment

Cerebrospinal fluid interferon alpha levels correlate with neurocognitive impairment in ambulatory HIV-Infected individuals

Accelerated epigenetic aging in brain is associated with pre-mortem HIV-associated neurocognitive disorders

Chemokines in cerebrospinal fluid correlate with cerebral metabolite patterns in HIV-infected individuals

Biomarkers of chemotaxis and inflammation in cerebrospinal fluid and serum in individuals with HIV-1 subtype C versus B

Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals