NDLI: Coupling scanning electron microscopy with DNA bar coding: a novel approach for thrips identification

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Coupling scanning electron microscopy with DNA bar coding: a novel approach for thrips identification

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Applied Entomology and Zoology : Volume 49, Issue 2, May 2014

Applied Entomology and Zoology : Volume 49, Issue 1, February 2014

Applied Entomology and Zoology : Volume 48

Applied Entomology and Zoology : Volume 47

Applied Entomology and Zoology : Volume 46

Coupling scanning electron microscopy with DNA bar coding: a novel approach for thrips identification

Content Provider	Springer Nature Link
Author	Kumar, Vivek Seal, Dakshina R. Osborne, Lance S. McKenzie, Cindy L.
Copyright Year	2014
Abstract	The small size and cryptic nature of thrips pests often make their monitoring and the identification process difficult. With the aim to obtain accurate identification of various pest species in the order Thysanoptera, a pilot study was conducted on an invasive thrips species Scirtothrips dorsalis Hood in Florida. The specific objective of this pilot study was to assess if the thrips specimens processed to be observed under scanning electron microscopy (SEM) can be further utilized for DNA based assays. Larvae and adults of S. dorsalis were subjected to traditional morphological identification using high resolution SEM prior to their DNA extraction. Sequence results of both mtCO1 and ITS rDNA of individual larva and adult S. dorsalis were found to be in agreement with the taxonomic identification conducted using SEM and each result confirmed the other technique. Our results suggest that steps involved during specimen preparation and observation under SEM does not impact DNA analysis of the sample. The two techniques together could be used for the correct identification of various thrips species using the same specimen. The proof of concept was further tested on three other important thrips species Frankliniella occidentalis (Pergande), F. schultzei (Trybom) and Thrips palmi Karny, which confirmed the robustness of the technique. The technique has broader significance for different areas under entomological sciences especially medical, forensic and taxonomic studies where the samples put under a high beam of ions for investigation are often required for DNA analysis.
Starting Page	403
Ending Page	409
Page Count	7
File Format	PDF
ISSN	00036862
Journal	Applied Entomology and Zoology
Volume Number	49
Issue Number	3
e-ISSN	1347605X
Language	English
Publisher	Springer Japan
Publisher Date	2014-04-12
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Scirtothrips dorsalis Thrips Morphological identification Electron microscope Entomology Agriculture Applied Ecology Plant Pathology Zoology Environmental Management
Content Type	Text
Resource Type	Article
Subject	Insect Science

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Occurrence of a novel strain of Scirtothrips dorsalis (Thysanoptera: Thripidae) in Japan and development of its molecular diagnostics

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Traditional and modern methods for the identification of thrips (Thysanoptera) species

Coupling scanning electron microscopy with DNA bar coding: a novel approach for thrips identification

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