NDLI: Potential to exploit postmortem enzyme degradation for evaluating arthropod viability

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A taxonomic issue of two species of Trissolcus (Hymenoptera: Platygastridae) parasitic on eggs of the brown-winged green bug, Plautia stali (Hemiptera: Pentatomidae): resurrection of T. plautiae, a cryptic species of T. japonicus revealed by morphology, reproductive isolation and molecular evidence

Changes of RNA virus infection rates and gut microbiota in young worker Apis mellifera (Hymenoptera: Apidae) of a chalkbrood-infected colony after a pollination task in a greenhouse

Coupling scanning electron microscopy with DNA bar coding: a novel approach for thrips identification

Survival of Bactrocera latifrons (Diptera: Tephritidae) adults under constant and fluctuating low temperatures

Potential to exploit postmortem enzyme degradation for evaluating arthropod viability

Ground beetle (Coleoptera: Carabidae) assemblages associated with a satoyama landscape in Japan: the effects of soil moisture, weed height, and distance from woodlands

Capability of Scolothrips takahashii (Thysanoptera: Thripidae) as a control agent of Tetranychus urticae (Acari: Tetranychidae) for protecting strawberry plug plants in summer

Development of a cell-based assay for ecdysteroid quantification using an early ecdysteroid-inducible gene promoter

Effect of entomopathogenic Aspergillus strains against the pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae)

Age-dependent changes in feeding preference and effects of commercial semi-synthetic diets on development of the legume pod borer, Maruca vitrata (Lepidoptera: Crambidae)

Movement efficiency and behavior of termites in tunnels with changing width

Seasonal occurrence of Laodelphax striatellus (Hemiptera: Delphacidae) in a rice-forage crops mixed cropping area in central Kyushu, Japan

Calcium hypochlorite for removing stalks on eggs of the green lacewing Chrysoperla genanigra (Neuroptera: Chrysopidae)

Isolation and characterization of 15 polymorphic microsatellite markers for the fig-pollinating wasp, Blastophaga nipponica (Hymenoptera: Agaonidae)

Isolation of nine polymorphic microsatellite loci from the burying beetle, Nicrophorus quadripunctatus (Coleoptera: Silphidae)

On the estimation of temperature-dependent development rate

Confuser$^{®}$ MM can be used as an attractant to enable monitoring of the peach leafminer moth, Lyonetia clerkella (Lepidoptera: Lyonetiidae), in peach orchards treated with a mating disrupter

Applied Entomology and Zoology : Volume 49, Issue 2, May 2014

Applied Entomology and Zoology : Volume 49, Issue 1, February 2014

Applied Entomology and Zoology : Volume 48

Applied Entomology and Zoology : Volume 47

Applied Entomology and Zoology : Volume 46

Potential to exploit postmortem enzyme degradation for evaluating arthropod viability

Content Provider	Springer Nature Link
Author	Phillips, Craig B. Iline, Ilia I. Novoselov, Max Richards, Nicola K.
Copyright Year	2014
Abstract	Inspecting for live organisms is the main method used to verify efficacy of phytosanitary treatments. Evaluating whether small, immobile organisms such as eggs, pupae and scale insects are alive or dead usually involves either checking morphological criteria or rearing them to observe development. These methods can be inaccurate, impractical and time consuming; thus, better methods are needed. To evaluate the potential for developing enzyme-based viability assays, we used electrophoretic gels to evaluate postmortem degradation of ten enzymes in Musca domestica L. (Diptera: Muscidae), four in Bemisia flocculosa Gill and Holder (Hemiptera: Aleyrodidae), and seven in Listronotus bonariensis (Kuschel) (Coleoptera: Curculionidae). Fresh insects displayed strong enzyme activity and distinct bands, but dead insects exhibited either no activity or weakened activity with reduced band resolution and increased migration of stained areas. Of ten enzymes investigated, seven showed clear indications of degradation just 1 day postmortem. Polyacrylamide gel electrophoresis of enzymes can be used to evaluate organism viability and has potential for estimating postmortem intervals. We also measured postmortem degradation rates of five M. domestica enzymes by assaying them in solution; these showed constant or gradually declining activity for 28 days postmortem, so live and dead specimens were less easily distinguished. By assaying enzymes in solution, it is possible to develop quick, easily operated tests that can be used outside the laboratory for a variety of quarantine-related purposes.
Starting Page	421
Ending Page	428
Page Count	8
File Format	PDF
ISSN	00036862
Journal	Applied Entomology and Zoology
Volume Number	49
Issue Number	3
e-ISSN	1347605X
Language	English
Publisher	Springer Japan
Publisher Date	2014-04-23
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Viability assessment Phytosanitary inspection Treatment efficacy Postmortem interval Biochemical assay Entomology Agriculture Applied Ecology Plant Pathology Zoology Environmental Management
Content Type	Text
Resource Type	Article
Subject	Insect Science

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Potential to exploit postmortem enzyme degradation for evaluating arthropod viability

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Potential to exploit postmortem enzyme degradation for evaluating arthropod viability

Forced hot-air treatment against Bactrocera papayae (Diptera: Tephritidae) in papaya

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