NDLI: First insights into the microbial diversity in the omasum and reticulum of bovine using Illumina sequencing

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First insights into the microbial diversity in the omasum and reticulum of bovine using Illumina sequencing

Content Provider	Springer Nature Link
Author	Peng, Shuai Yin, Jigang Liu, Xiaolei Jia, Boyin Chang, Zhiguang Lu, Huijun Jiang, Ning Chen, Qijun
Copyright Year	2015
Abstract	The digestive systems of mammals harbor a complex gut microbiome, comprising bacteria and other microorganisms that confer metabolic and immunological benefits to the host. Ruminants that digest plant-based foods have a four-compartment stomach consisting of the rumen, reticulum, omasum, and abomasum. The microorganisms in the stomach are essential for providing the host with critical nutrients. However, the majority of these microorganisms are unknown species. The microbiome of the stomach is diverse, and the majority of these organisms cannot be cultured. Next-generation sequencing (NGS) combined with bioinformatic analysis tools have allowed the dissection of the composition of the microbiome in samples collected from a specific environment. In this study, for the first time, the bacterial composition in two compartments, the reticulum and the omasum, of bovine were analyzed using a metagenomic approach and compared to the bacterial composition of the rumen. These data will assist in understanding the biology of ruminants and benefit the agricultural industry. The diversity and composition of the bacterial community in samples collected from the rumen, reticulum, and omasum of bovines in the Changchun Region of Northeast China were analyzed by sequencing the V3 region of the 16S rRNA gene using a barcoded Illumina paired-end sequencing technique, and the primary composition of the microbiome in the rumen, reticulum, and omasum of the bovines was determined. These microbiomes contained 17 phyla and 107 genera in all three samples. Five phyla, Bacteroidetes, Firmicutes, Proteobacteria, Spirochaetes, and Lentisphaerae, were the most abundant taxonomic groups. Additionally, the different stomach compartments harbored different compositions of the microorganisms.
Starting Page	393
Ending Page	401
Page Count	9
File Format	PDF
ISSN	12341983
Journal	Journal of Applied Genetics
Volume Number	56
Issue Number	3
e-ISSN	21903883
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-01-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Illumina Microbiome diversity Omasum Rumen Reticulum V3 region of the 16S rRNA Life Sciences Animal Genetics and Genomics Human Genetics Microbial Genetics and Genomics Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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