NDLI: Analysis of the wheat endosperm transcriptome

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Journal of Applied Genetics : Volume 47, Issue 3, September 2006

Journal of Applied Genetics : Volume 47, Issue 2, June 2006

Journal of Applied Genetics : Volume 47, Issue 1, March 2006

Analysis of the wheat endosperm transcriptome

Content Provider	Springer Nature Link
Author	Laudencia Chingcuanco, Debbie L. Stamova, Boryana S. Lazo, Gerard R. Cui, Xiangqin Anderson, Olin D.
Copyright Year	2006
Abstract	Among the cereals, wheat is the most widely grown geographically and is part of the staple diet in much of the world. Understanding how the cereal endosperm develops and functions will help generate better tools to manipulate grain qualities important to end-users. We used a genomics approach to identify and characterize genes that are expressed in the wheat endosperm. We analyzed the 17 949 publicly available wheat endosperm EST sequences to identify genes involved in the biological processes that occur within this tissue. Clustering and assembly of the ESTs resulted in the identification of 6 187 tentative unique genes, 2 358 of which formed contigs and 3 829 remained as singletons. A BLAST similarity search against the NCBI non-redundant sequence database revealed abundant messages for storage proteins, putative defense proteins, and proteins involved in starch and sucrose metabolism. The level of abundance of the putatively identified genes reflects the physiology of the developing endosperm. Half of the identified genes have unknown functions. Approximately 61% of the endosperm ESTs has been tentatively mapped in the hexaploid wheat genome. Using microarrays for global RNA profiling, we identified endosperm genes that are specifically up regulated in the developing grain.
Starting Page	287
Ending Page	302
Page Count	16
File Format	PDF
ISSN	12341983
Journal	Journal of Applied Genetics
Volume Number	47
Issue Number	4
e-ISSN	21903883
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	endosperm EST mapping microarray transposon wheat Life Sciences Animal Genetics and Genomics Human Genetics Microbial Genetics and Genomics Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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