NDLI: ΔNp63α attenuates tumor aggressiveness by suppressing miR-205/ZEB1-mediated epithelial–mesenchymal transition in cervical squamous cell carcinoma

Content Provider	Springer Nature Link
Author	Zhao, Weidong Wang, Huiyan Han, Xiaohui Ma, Jie Zhou, Yuanyuan Chen, Zhengzheng Zhou, Hu Xu, Hanjie Sun, Zhengwei Kong, Beihua Fang, Huiying
Copyright Year	2016
Abstract	Cervical cancer is one of the most common female cancers worldwide. Although the therapeutic outcomes of patients with early-stage cervical cancer have been significantly improved in the past decades, tumor metastasis and recurrence remain the major causes of cervical cancer-related deaths. In cervical squamous cell carcinoma (SCC), the aberrant activation of epithelial–mesenchymal transition (EMT), a crucial process in invasion and metastasis of epithelial cancer, could promote lymph nodal metastasis and recurrence, and predicts poor prognosis. In this study, we show that the expression levels of EMT markers, β-catenin and Vimentin, are associated with the p63 isoform ΔNp63α in SCC by using immunohistochemistry staining and analysis. Compared to the control SiHa cells (SiHa-NC), the expression of E-cadherin and β-catenin are upregulated, while Vimentin and ZEB1 are downregulated in the constructed SiHa cell line with stable ΔNp63α overexpression (SiHa-ΔNp63α). Besides, the migration and invasion abilities are also suppressed in SiHa-ΔNp63α cells with a typical epithelial morphology with cobblestone-like shape, suggesting that ΔNp63α is a vital EMT repressor in SCC cells. In addition, the involvement of miR-205/ZEB1 axis in the inhibition effect of ΔNp63α on EMT program is revealed by a miRNA array and confirmed by the subsequent transfection of the miR-205 mimic and antagomir. Moreover, SCC patients with low ΔNp63α expression and high EMT level show more frequent metastasis and recurrence as well as reduced overall survival. Therefore, EMT program and its vital repressor ΔNp63α could be used as biomarkers for tumor metastasis and recurrence in cervical cancer.
Starting Page	10621
Ending Page	10632
Page Count	12
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	37
Issue Number	8
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-02-10
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	ΔNp63α Epithelial–mesenchymal transition Cervical squamous cell carcinoma β-catenin Vimentin Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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