NDLI: MicroRNA-27b exerts an oncogenic function by targeting Fbxw7 in human hepatocellular carcinoma

Content Provider	Springer Nature Link
Author	Sun, Xin Fang Sun, Jin Ping Hou, Hong Tao Li, Ke Liu, Xin Ge, Quan Xing
Copyright Year	2016
Abstract	Aberrant expression of microRNAs (miRNAs) plays fundamental effect on the pathogenesis of hepatocellular carcinoma (HCC). MiR-27b was previously found to play important roles in human cancers. However, its expression status, clinical significance, and biological functions in HCC remain largely unclear. The expression status of miR-27b in HCC specimens and cells were determined with qRT-PCR. MTT, 5-bromodeoxyuridine (BrdU) proliferation assays, and flow cytometry analysis were carried out to assay proliferation, cell cycle, and apoptosis. A subcutaneous model was used to evaluated the HCC tumor growth in vivo. The putative target gene of miR-27b was disclosed by TargetScan and a luciferase reporter assay. The levels of miR-27b were overexpressed in HCC. Overexpression of miR-27b was correlated with adverse prognostic features and reduced survival rate. Inhibition of miR-27b in SMMC-7721 cells remarkably suppressed proliferative ability and cell-cycle progression while enhanced apoptosis. In contrast, miR-27b overexpression resulted in prominent increased proliferation and process of cell cycle and reduced apoptosis of Hep3B cells. In vivo studies showed that knockdown of miR-27b inhibited the in vivo growth of SMMC-7721 cells in mouse xenograft model. Furthermore, we confirmed that Fbxw7 was directly regulated by miR-27b and mediated the roles of miR-27b in HCC. We suggest that miR-27b serves as an oncogenic miRNA in HCC by modulating proliferation, cell-cycle progression, and apoptosis, and its oncogenic effect is mediated by its downstream target gene, Fbxw7.
Starting Page	15325
Ending Page	15332
Page Count	8
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	37
Issue Number	11
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-10-04
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	MicroRNA-27b Hepatocellular carcinoma Cell growth Oncogenic microRNA Fbxw7 Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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