NDLI: Exosomes mediate drug resistance transfer in MCF-7 breast cancer cells and a probable mechanism is delivery of P-glycoprotein

Content Provider	Springer Nature Link
Author	Lv, Meng meng Zhu, Xing ya Chen, Wei xian Zhong, Shan liang Hu, Qing Ma, Teng fei Zhang, Jun Chen, Lin Tang, Jin hai Zhao, Jian hua
Copyright Year	2014
Abstract	Acquired drug resistance is a major obstacle to chemotherapy of cancers. In this study, we aim to investigate the role of exosomes in drug-resistance transfer between breast cancer cells and detect the probable mechanism. A docetaxel-resistant variant of MCF-7 cell line (MCF-7/DOC) was established and then compared with the drug-sensitive variant (MCF-7/S). Exosomes were expelled from the cell supernatant using ultracentrifugation. Drug resistance was assessed by apoptosis assay and MTT examination. Expressions of P-glycoprotein (P-gp) were analyzed by flow cytometry. Stained exosomes were absorbed by receipt cells. MCF-7/S in the presence of exosomes extracted from the supernatant of MCF-7/DOC (DOC/exo) acquired drug resistance, while MCF-7/S exposed to their own exosomes (S/exo) did not. P-gp expression patterns of exosomes were similar as the originated cells. P-gp expression of MCF-7/S increased after incubation with DOC/exo and was affected by the amount of exosomes. Exosomes are effective in transferring drug resistance as well as P-gp from drug-resistant breast cancer cells to sensitive ones. The delivery of P-gp via exosomes may be a mechanism of exosome-mediated drug resistance transfer.
Starting Page	10773
Ending Page	10779
Page Count	7
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	11
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-07-31
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	P-glycoprotein Exosomes Drug-resistance Docetaxel Transfer Breast cancer Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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