NDLI: Assessment of DNA damage caused by locally produced hydroxyapatite-silica nanocomposite using Comet assay on human lung fibroblast cell line

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Assessment of DNA damage caused by locally produced hydroxyapatite-silica nanocomposite using Comet assay on human lung fibroblast cell line

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Molecular & Cellular Toxicology : Volume 7

Molecular & Cellular Toxicology : Volume 6

Assessment of DNA damage caused by locally produced hydroxyapatite-silica nanocomposite using Comet assay on human lung fibroblast cell line

Content Provider	Springer Nature Link
Author	Musa, Marahaini Kannan, Thirumulu Ponnuraj Masudi, Sam’an Malik Rahman, Ismail Ab
Copyright Year	2012
Abstract	The growing interest of nanotechnology in dentistry has sparked various applications of biomaterials in nanoscale to be developed. The aim of this study was to evaluate the genotoxicity effect of locally produced hydroxyapatite-silica nanocomposite (School of Dental Sciences, Universiti Sains Malaysia, Malaysia) using Comet assay on human lung fibroblast cell line, MRC-5. Extraction of this test material was prepared and the concentrations which produced IC$_{10}$, IC$_{25}$ and IC$_{50}$ in cytotoxicity tests (MTT assay) were recorded. Three specific concentrations, 0.00005 g/mL, 0.0009 g/mL and 0.1 g/mL for IC$_{10}$, 1C$_{25}$ and IC$_{50}$ respectively were further used to evaluate the genotoxicity effect along with concurrent positive (hydrogen peroxide) and negative (Eagle’s Minimum Essential Medium) controls. There was no significant difference in the tail moments between negative control and treatment groups (0.00005 g/mL). Dose-dependent relationship was observed, where significant difference was noticed in the tail moments between 0.0009 g/mL and 0.1 g/mL groups with that of the negative control. However, since the values were still less than 5, it can be considered as non-genotoxic. The tail moments between different concentrations of hydroxyapatite-silica nanocomposite and positive control differed significantly (P/0.05). This concludes that the locally produced HAsilica nanocomposite is non-genotoxic by Comet assay under the present test conditions.
Starting Page	53
Ending Page	60
Page Count	8
File Format	PDF
ISSN	1738642X
Journal	Molecular & Cellular Toxicology
Volume Number	8
Issue Number	1
e-ISSN	20928467
Language	English
Publisher	The Korean Society of Toxicogenomics and Toxicoproteomics
Publisher Date	2012-04-04
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hydroxyapatite-silica nanocomposite MTT assay Comet assay DNA damage Genotoxicity Pharmacology/Toxicology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health Toxicology Pathology and Forensic Medicine

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