NDLI: Comparative evaluation of 16S rRNA gene in world-wide strains of Streptococcus iniae and Streptococcus parauberis for early diagnostic marker

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Comparative evaluation of 16S rRNA gene in world-wide strains of Streptococcus iniae and Streptococcus parauberis for early diagnostic marker

Content Provider	Springer Nature Link
Author	Mishra, Anshuman Nam, Gyu Hwi Gim, Jeong An Seong, Minji Choe, Yunjeong Lee, Hee Eun Jo, Ara Kim, Suhkmann Kim, Do Hyung Cha, Hee Jae Kang, Ho Young Choi, Yung Hyun Kim, Heui Soo
Copyright Year	2017
Abstract	Two bacterial etiological agents of the disease, Streptococcus iniae and Streptococcus parauberis has been associated with fish mortalities and heavy economic loss in all over the world. Bacterial identification based on 16S rRNA sequencing is very fast, accurate and reliable in comparison to other traditional phenotype methodologies. In this study, we investigate the usefulness of this method for diagnosis and identification of Streptococcus species. We have selected 61 phylogeographic strains of Streptococcus (34 strains of S. iniae and 27 strains of S. parauberis) and designed the universal primer against the identified most hypervariable region of the 16S rRNA gene. Our universal primer able to identify any geographical strains and offers a useful and fast alternative in a clinical laboratory under routine conditions. Based on our studies, we have developed an algorithm for appropriate control of S. iniae and S. parauberis disease. We suggested the phenotype observation along with universal primer combination to detect any kind of infection or carriers at early stages.
Starting Page	779
Ending Page	791
Page Count	13
File Format	PDF
ISSN	19769571
Journal	Genes & Genomics
Volume Number	39
Issue Number	7
e-ISSN	20929293
Language	English
Publisher	The Genetics Society of Korea
Publisher Date	2017-05-30
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phylogenetic tree Geography Sequencing Streptococcus iniae Streptococcus parauberis Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Biochemistry Molecular Biology

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