NDLI: Hidden stressors in the clonogenic assay used in radiobiology experiments

Content Provider	Springer Nature Link
Author	Potter, M. D. E. Suchowerska, N. Rizvi, S. McKenzie, D. R.
Copyright Year	2011
Abstract	While clonogenic assays are extensively used in radiobiology, there is no widely accepted procedure for choosing the composition of the cell culture media. Cell line suppliers recommend a specific culture medium for each cell line, however a researcher will frequently customize this aspect of the protocol by supplementing the recommended support medium with additives. For example, many researchers add antibiotics, in order to avoid contamination of cells and the consequent loss of data, with little discussion of the influence of the antibiotics on the clonogenic survival of the cells. It is assumed that the effect of any variables in the growth medium on cell survival is taken into consideration by comparing the survival fraction relative to that of controls grown under the same conditions. In the search for better cancer treatment, the effect of various stressors on clonogenic cell survival is under investigation. This study seeks to identify and test potential stressors commonly introduced into the cell culture medium, which may confound the response to radiation.
Starting Page	345
Ending Page	350
Page Count	6
File Format	PDF
ISSN	01589938
Journal	Australasian Physics & Engineering Sciences in Medicine
Volume Number	34
Issue Number	3
e-ISSN	18795447
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-06-21
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Clonogenic assay Bystander effect Cell culture media Cell growth stressors Radiobiology Biophysics and Biological Physics Medical and Radiation Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Radiology, Nuclear Medicine and Imaging Physics and Astronomy Biophysics Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Hidden stressors in the clonogenic assay used in radiobiology experiments.

Non-targeted effects of ionising radiation and radiotherapy

Optimisation of exposure conditions for in vitro radiobiology experiments

Optimisation of exposure conditions for in vitro radiobiology experiments.

Applied radiobiology: continuous irradiation and brachytherapy

Non-targeted effects of ionising radiation and radiotherapy.

A percolation-like model for simulating inter-cellular diffusion in the context of bystander signalling in tumour

Loredana Marcu, Eva Bezak (eds): Recent advances and research updates, medical physics and radiobiology

Applied radiobiology and bioeffect planning by David R. Wigg

Hidden stressors in the clonogenic assay used in radiobiology experiments

Similar Documents

Hidden stressors in the clonogenic assay used in radiobiology experiments.

Non-targeted effects of ionising radiation and radiotherapy

Optimisation of exposure conditions for in vitro radiobiology experiments

Optimisation of exposure conditions for in vitro radiobiology experiments.

Applied radiobiology: continuous irradiation and brachytherapy

Non-targeted effects of ionising radiation and radiotherapy.

A percolation-like model for simulating inter-cellular diffusion in the context of bystander signalling in tumour

Loredana Marcu, Eva Bezak (eds): Recent advances and research updates, medical physics and radiobiology

Applied radiobiology and bioeffect planning by David R. Wigg

Hidden stressors in the clonogenic assay used in radiobiology experiments