NDLI: Optimisation of exposure conditions for in vitro radiobiology experiments

Content Provider	Springer Nature Link
Author	Claridge Mackonis, Elizabeth Suchowerska, Natalka Naseri, Pourandokht McKenzie, David R.
Copyright Year	2012
Abstract	Despite the long history of using cell cultures in vitro for radiobiological studies, there is to date no study specifically addressing the dosimetric implications of flask selection and exposure environment in clonogenic assays. The consequent variability in dosimetry between laboratories impedes the comparison of results. In this study we compare the dose to cells adherent to the base of three types of commonly used culture flasks or plates. The cells are exposed to a 6MV clinical photon beam using either an open or a half blocked field. The depth of medium in each flask is varied with the medium surrounding the flask either water or air. The results show that the dose to the cells is more affected by the scattering conditions surrounding the flasks than by the level of filling within the flask. It is recommended that water or a water equivalent phantom material is used to surround the flasks or plates to approximate full scatter conditions at the cell layer. However for modulated fields, surrounding the 24 well plates with water-equivalent material is inadequate because of the large volume of air surrounding individual wells. Our results stress the importance of measuring the dose for new experimental configurations.
Starting Page	151
Ending Page	157
Page Count	7
File Format	PDF
ISSN	01589938
Journal	Australasian Physics & Engineering Sciences in Medicine
Volume Number	35
Issue Number	2
e-ISSN	18795447
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-03-28
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Radiobiology Dosimetry In vitro Irradiation MV Biomedical Engineering Medical and Radiation Physics Biomedicine general Biophysics and Biological Physics
Content Type	Text
Resource Type	Article
Subject	Radiology, Nuclear Medicine and Imaging Physics and Astronomy Biophysics Biomedical Engineering

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