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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kaur Sandhu, Simanjeet Singh Oberoi, Harinder Singh Dhaliwal, Sandeep Babbar, Neha Kaur, Ujjal Nanda, Dhiraj Kumar, Dinesh |
| Copyright Year | 2011 |
| Abstract | The aim of this study was to assess the potential of using the crude filtrate extract (CFE) produced by a newly isolated strain of Aspergillus oryzae and fermentation with a novel thermotolerant strain of Pichia kudriavzevii for the production of ethanol from kinnow peel waste (KP) via simultaneous saccharification and fermentation (SSF). High-performance liquid chromatography determination showed that pre-hydrolysis of KP with CFE at 3 cellulase filter paper units/g dry substrate (FPU/g-ds) at 50°C resulted in 24.87 ± 0.75 g l$^{-1}$ glucose, 21.98 ± 0.53 g l$^{-1}$ fructose, 10.86 ± 0.34 g l$^{-1}$ sucrose and 6.56 ± 0.29 g l$^{-1}$ galacturonic acid (GA) along with insignificant amounts of arabinose, galactose and xylose. Simultaneous saccharification and fermentation of hydrothermally pretreated KP at a substrate concentration of 15% (w v$^{-1}$) was conducted in a 2.5-l laboratory fermentor with P. kudriavzevii at 40°C after a 3-h pre-hydrolysis. Oligosaccharides were not produced during the SSF process. Ethanol production leveled off after 12 h, resulting in an ethanol concentration and productivity of 33.87 g l$^{-1}$ and 2.82 g l$^{-1}$ h$^{-1}$, respectively. These results demonstrate the potentiality of SSF using crude enzymes and P. kudriavzevii for the scale-up production of ethanol from KP. |
| Starting Page | 655 |
| Ending Page | 666 |
| Page Count | 12 |
| File Format | |
| ISSN | 15904261 |
| Journal | Annals of Microbiology |
| Volume Number | 62 |
| Issue Number | 2 |
| e-ISSN | 18692044 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2011-07-08 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Aspergillus oryzae Ethanol Pichia kudriavzevii Kinnow peels Simultaneous saccharification and fermentation Microbial Genetics and Genomics Fungus Genetics Microbiology Microbial Ecology Applied Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Applied Microbiology and Biotechnology |
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