NDLI: Expression of mitochondrial malate dehydrogenase in Escherichia coli improves phosphate solubilization

Content Provider	Springer Nature Link
Author	Lü, Jun Gao, Xiaorong Dong, Zhimin An, Lijia
Copyright Year	2011
Abstract	Penicillium oxalicum C2 was isolated from the rhizosphere of Mexican stonecrop based on its high level of tricalcium phosphate solubilizing activity. A full-length gene encoding mitochondrial malate dehydrogenase (mMDH) was cloned from P. oxalicum C2 by RT-PCR and RACE techniques. The cDNA was 1,284 bp in length, with a complete open reading frame of 1,023 bp encoding a protein of 340 amino acids with a predicted molecular mass of 35.7 kDa. The predicted amino acid sequence shared high identity with mMDHs from other organisms. Escherichia coli strain BL21(DE3) transformed with the P. oxalicum C2 mMDH gene showed an increase in MDH activity of about 5-fold compared to non-transformed cell as measured in cell extracts. It also secreted more malate, lactate, acetate, citrate, oxalate and produced higher clearing halos when grown in plates with tricalcium phosphate as the sole source of phosphate. These results showed that expression of the P. oxalicum C2 mMDH gene in E. coli could enhance organic acid secretion and improve the phosphate solubilizing ability of the cell.
Starting Page	607
Ending Page	614
Page Count	8
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	62
Issue Number	2
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-07-08
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Phosphate solubilizing Penicillium oxalicum Malate dehydrogenase Organic acid Microbial Genetics and Genomics Fungus Genetics Microbiology Microbial Ecology Applied Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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