NDLI: Seed-borne mycoflora of alfalfa (Medicago sativa L.) in the Riyadh Region of Saudi Arabia

Content Provider	Springer Nature Link
Author	Al Askar, Abdul Aziz A. Ghoneem, Khalid M. Rashad, Younes M.
Copyright Year	2011
Abstract	Fifteen seed samples of local alfalfa (Medicago sativa L.) cultivar (Hegazy) collected from fields at different governorates of the Riyadh region in Saudi Arabia were screened for their seed-borne mycoflora. Standard moist blotter and deep-freezing blotter methods recommended by the International Seed Testing Association, in addition to an innovative alkaline seed-bed method were applied. A total of 24 genera and 35 species of fungi were isolated using the above-mentioned techniques. Alternaria alternata, Cladosporium sp., Aspergillus sp., Stemphylium sp. and Penicillium sp. were the genera most commonly isolated. Among the various techniques adopted for detection, the alkaline NaOH method was found to be effective and yielded the maximum number of pathogenic fungi. Syndromes of seed discoloration were also investigated. The alkaline seed-bed method using NaOH was used to detect saprophytic and pathogenic seed-borne fungi associated with discolored seeds. A total of 15 genera and 26 species of fungi were isolated. Cladosporium sp., Alternaria alternata and Aspergillus species were the saprophytic species detected most, while Stemphylium botryosum and Fusarium incarnatum were common pathogenic fungi found on discolored seeds. The average of incidence and occurrence percentages of most detected fungi were higher in discolored seeds than in normal seeds. Seed germination was also affected significantly by discoloration. The data also indicate that seed discoloration decreases seed germination significantly (by 26.3–60%).
Starting Page	273
Ending Page	281
Page Count	9
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	62
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-05-08
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Alfalfa Germination Seed-borne fungi and seed discoloration Applied Microbiology Medical Microbiology Microbiology Fungus Genetics Microbial Ecology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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