NDLI: Molecular characterization of plasmid-encoded ACC-1a4 β-lactamase produced by Salmonella strains isolated from water in a Tunisian hospital

Content Provider	Springer Nature Link
Author	Ben Aissa, Melek Herteg Fernea, Adriana Bouraoui, Hanene Ben Aissa, Ridha Mercuri, Paola Sandra Manai, Mohamed
Copyright Year	2011
Abstract	A collection of non-typhoidal Salmonella strains, designated MMB1 to MMB18, resistant to third generation cephalosporins were isolated from water in a Tunisian hospital. Isolates harbored a new bla $_{ACC}$ gene located on a small transmissible plasmid of 5 kb. The ACC-1a4 enzyme was produced in Escherichia coli, purified and characterized. Compared to the chromosomal bla $_{ACC-1a}$ gene, the bla $_{ ACC-1a4 }$ exhibit three mutations. We determined the kinetic parameters of our enzyme for a representative series of β-lactam substrates. The ACC-1a4 enzyme was active on penicillins and cephalosporins, nitrocefin and cephalothin being its best tested substrates. This is a real cause for alarm since these mutations showed that the DNA sequence of bla $_{ACC}$ is an intermediate between those of bla $_{ ACC-1a }$ and bla $_{ ACC-4 }$ and might increase the probability of the appearance and spreading of ACC-4-mediated resistance in the hospital environment.
Starting Page	31
Ending Page	36
Page Count	6
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	62
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-02-19
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Salmonella β-lactamase ACC-1a4 Kinetic Applied Microbiology Medical Microbiology Microbiology Fungus Genetics Microbial Ecology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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