NDLI: Molecular detection and community analysis of arbuscular mycorrhizal fungi in the rhizosphere of Phellodendron amurense

Content Provider	Springer Nature Link
Author	Jie, Weiguang Cai, Baiyan Ge, Jingping
Copyright Year	2012
Abstract	Arbuscular mycorrhizal fungi were isolated from rhizospheric soil samples collected from Phellodendron amurense. Nested-PCR was conducted to amplify NS31/Glol domain sequences of the small-subunit (18S) rDNA from fungi in the collected samples. The amplified products were subsequently analyzed by denaturing gradient gel electrophoresis (DGGE). DGGE results were analyzed in conjunction with sequencing and phylogenetic data to investigate the composition of different fungal strains present in the AM communities of P. amurense. Four AM fungal species were identified, including Glomus intraradices, G. mosseae, G. versiforme and Scutellospora calospora. Glomus versiforme, however, was not detected in the root samples by nested-PCR, suggesting the roots of P. amurense are not colonized by this species. The DGGE profiles of the rhizospheric soil and root samples differed, suggesting that the roots and soil support different fungal communities. Sequences could be clustered into three groups, viz. Glomus, Scutellospora and Hyponectria buxi. Glomus was determined to be the prevalent AM fungi in the root and rhizospheric soil.
Starting Page	1769
Ending Page	1777
Page Count	9
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	62
Issue Number	4
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-02-29
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Phellodendron amurense·25S rDNA 18S rDNA Nested-PCR Denaturing gradient gel electrophoresis Microbial Genetics and Genomics Medical Microbiology Applied Microbiology Microbiology Microbial Ecology Fungus Genetics
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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