NDLI: Isolation of phaC gene from marine bacteria Paracoccus homiensis strain E33 by magnetic beads subtractive hybridization

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Isolation of phaC gene from marine bacteria Paracoccus homiensis strain E33 by magnetic beads subtractive hybridization

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Isolation of phaC gene from marine bacteria Paracoccus homiensis strain E33 by magnetic beads subtractive hybridization

Content Provider	Springer Nature Link
Author	Latisnere Barragan, Hever López Cortés, Alejandro
Copyright Year	2012
Abstract	The isolation of unknown DNA sequences is an important task in molecular biology research. The subtractive hybridization by magnetic beads technique reduces time and cost when the aim of the study is to isolate new genes. We have developed an alternative strategy that combines the subtractive hybridization method with an enrichment microsatellite strategy to isolate a de novo DNA sequence of the phaC gene involved in the polymerization of polyhydroxyalkanoates. A 113-bp fragment was developed as a probe and, based on its melting temperature calculated using the nearest-neighbor model, used to isolate the full-length phaC gene (GenBank Accession number HM026759) of the marine bacteria Paracoccus homiensis strain E33, which was isolated from polluted microbial mats.
Starting Page	1691
Ending Page	1695
Page Count	5
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	62
Issue Number	4
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-02-22
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Subtractive hybridization Polyhydroxyalkanoate Library enrichment Nearestneighbor model Microbial Genetics and Genomics Medical Microbiology Applied Microbiology Microbiology Microbial Ecology Fungus Genetics
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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