NDLI: Optimization of an inducible, chromosomally encoded benzo [a] pyrene (BaP) degradation pathway in Bacillus subtilis BMT4i (MTCC 9447)

Content Provider	Springer Nature Link
Author	Lily, Madhuri Kaushish Bahuguna, Ashutosh Dangwal, Koushalya Garg, Veena
Copyright Year	2010
Abstract	Benzo [a] pyrene (BaP), a pentacyclic polyaromatic hydrocarbon, is 1 of the 12 target compounds defined in the new US Environmental Protection Agency strategy for controlling persistent, bioaccumulative, and toxic pollutants. We previously isolated a novel strain Bacillus subtilis BMT4i capable of utilizing BaP as sole source of carbon and energy. The present study investigated (1) whether the BaP degradation pathway is inducible, and (2) whether it is plasmid-encoded. Furthermore, physical (temperature, pH, and UV-induced photolysis of BaP) and chemical (BaP concentration, surfactant, and ionic strength) parameters for BaP degradation were determined. Our findings revealed a ten-fold enhanced degradation rate in induced vs non-induced culture in the presence of chloramphenicol, suggesting that the BaP degradation pathway is inducible. Physical methods demonstrated the lack of plasmid in BMT4i—a result further complimented by plasmid curing, which had no effect on BaP degradability thus a chromosomal localization can be inferred. Maximum BaP degradation in BMT4i was observed under the following physical and chemical conditions: 30°C, pH 8.0, UV-induced photolysis of BaP-basal salt mineral medium (BSM), 150 μg/ml BaP, 0.01% Tween-20, and 400–1,800 μM MgSO$_{4}$. These conditions could be beneficial in the development and standardization of effective bioremediation protocols using B. subtilis BMT4i.
Starting Page	51
Ending Page	58
Page Count	8
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	60
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-01-27
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Bacillus subtilis BMT4i Benzo [a] pyrene Biodegradation High performance liquid chromatography Plasmid curing Applied Microbiology Medical Microbiology Fungus Genetics Microbial Ecology Microbial Genetics and Genomics Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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