NDLI: Diversity analysis of nitrite reductase genes (nirS) in black soil under different long-term fertilization conditions

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Annals of Microbiology : Volume 59

Annals of Microbiology : Volume 58

Annals of Microbiology : Volume 57

Annals of Microbiology : Volume 56

Diversity analysis of nitrite reductase genes (nirS) in black soil under different long-term fertilization conditions

Content Provider	Springer Nature Link
Author	Tang, Hui Yan, Kai Zhang, Liping Chi, Fengqin Li, Qing Lian, Shujuan Wei, Dan
Copyright Year	2010
Abstract	A 30-year fertilizer experiment was conducted to investigate the effects of organic manure and mineral NPK fertilizer on black soil. Four experimental treatments were used: CK, organic manure, mineral NPK fertilizer, and organic manure plus mineral fertilizer. The diversity of the nirS gene was analyzed by PCR-restriction fragment length polymorphism. The Shannon diversity index and Evenness factor, Simpson’s diversity and Shannon-Weiner indices, principal component analysis and a one-way analysis of similarities test showed that organic manure made the opposite contribution to soil diversity of nirS genes compared to mineral fertilizer treatment, which resulted in increased diversity of the nirS gene. Comparison of the phylogenetic analysis of nirS genes in the mineral fertilizer and organic manure clone libraries showed that induction by mineral fertilizer was stronger than that by organic manure, based on the types of unique nirS genotypes found in the two libraries and the evolutionary distances between them.
Starting Page	97
Ending Page	104
Page Count	8
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	60
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-01-27
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Long-term fertilization Diversity nirS genes Applied Microbiology Medical Microbiology Fungus Genetics Microbial Ecology Microbial Genetics and Genomics Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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