NDLI: Parenteral exposure to DEHP and its effect on the microstructure of bone and Wnt signaling pathway in F2 female mice

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Parenteral exposure to DEHP and its effect on the microstructure of bone and Wnt signaling pathway in F2 female mice

Content Provider	Springer Nature Link
Author	Cheon, Keun Young Kil, Ki Hyun Choi, Jung In Kim, Ra Yon Kim, Mee Ran Kim, Jang Heub Cho, Hyun Hee
Copyright Year	2016
Abstract	Diethylhexyl phthalate (DEHP) is a widely used plasticizer, and its exposure via the enteral and parenteral routes can affect estrogen function and has transgenerational effect. The bones are the most important target organs for estrogen; however, the related gene pathway and the effects of DEHP on bones remain unknown. In this study, we evaluated whether parenteral exposure to DEHP has effects on bone microarchitecture in F2 mice, and determined the related gene pathway.Pregnant mice (F0) were exposed to DEHP, at doses of 30 μg/kg/day (low dose) and 400 μg/kg/day (high dose), and corn oil during pregnancy and lactation. F2 siblings were not treated with DEHP. We performed micro-CT to analyze the microstructure of the tibial head and microarray analysis on ovary cells from F2 female siblings of 17-18 weeks of age to evaluate the gene expression.The percentage of bone volume in the low- (5.55%± 0.85%) and high-dose (7.09%±4.15%) groups was higher than that in the control (2.25%±1.22%; P< 0.05). Trabecular number (per mm2) was higher in the low- (0.87±0.12) and high-dose (0.95±0.55) groups compared to that in the control (0.37±0.21; P<0.05). Trabecular thickness was greater in the high-dose group (0.073±0.006 μm) than in the control (0.060±0.002 μm) and low-dose (0.063±0.004 μm) groups. The structure model index (SMI) of the low- and high-dose groups was significantly lower than the corresponding value in the control group (P<0.05). Micro-CT analysis indicated proliferative changes in trabecular bone in the treated F2 siblings. In the microarray analysis of ovary cells, CTNNB1 (catenin beta 1) and PPP3R1 gene expressions were significantly modified in the low- and high-dose groups. SFRP1, DVL2, WNT11, and WNT16 showed significant expression changes in the low-dose group, but not in the high-dose group. Taken together, parenteral exposure to DEHP during pregnancy and lactation could influence the trabecular bone microarchitectures of F2 siblings and affect certain genes associated with the Wnt signaling pathway.
Starting Page	233
Ending Page	240
Page Count	8
File Format	PDF
ISSN	19760280
Journal	BioChip Journal
Volume Number	10
Issue Number	3
e-ISSN	20927843
Language	English
Publisher	The Korean BioChip Society (KBCS)
Publisher Date	2016-07-29
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DEHP Parenteral exposure Bone microstructure WNT pathway Microarray Biotechnology Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomedical Engineering Electrical and Electronic Engineering Biotechnology

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Parenteral exposure to DEHP and its effect on the microstructure of bone and Wnt signaling pathway in F2 female mice

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