NDLI: Micro and nanotechnology for early diagnosis and detection of rheumatic diseases-molecular markers

Please wait, while we are loading the content...

Development of a matrix metalloproteinase-2 (MMP-2) biosensing system by integrating an enzyme-mediated color development reaction into a common electronics components setup

A portable electromagnetic induction heating device for point-of-care diagnostics

Tumour-like druggable gene expression pattern of CaCo2 cells in microfluidic chip

Parenteral exposure to DEHP and its effect on the microstructure of bone and Wnt signaling pathway in F2 female mice

Quorum sensing is crucial to Escherichia coli O157:H7 biofilm formation under static or very slow laminar flow conditions

BioChip Journal : Volume 10, Issue 2, June 2016

BioChip Journal : Volume 10, Issue 1, March 2016

BioChip Journal : Volume 9

BioChip Journal : Volume 8

BioChip Journal : Volume 7

BioChip Journal : Volume 6

BioChip Journal : Volume 5

BioChip Journal : Volume 4

Micro and nanotechnology for early diagnosis and detection of rheumatic diseases-molecular markers

Content Provider	Springer Nature Link
Author	Marulli, Elisabetta Aloisi, Alessandra Di Giuseppe, Paolo Rinaldi, Rosaria
Copyright Year	2016
Abstract	Cytokine proteins are known as biomarker molecules, characteristic of a disease or specific body condition. Monitoring of the cytokine pattern in body fluids contributes to the diagnosis of rheumatic diseases associated with rheumatoid factor. Here, the proinflammatory cytokine Tumor Necrosis Factor alpha (TNF-α) was chosen as the first target of interest. We report on the development of a fast sandwich immunosorbent assay: anti-TNF-α antibody (Ab) immobilization on a pre-activated 3D micro-structured glass slide, coupled with direct fluorescence readout method. Two differently functionalized glass - Ab-immobilization substrates - were compared: O2 PLASMA/APTES activated glass - having a two-dimensional structure-, and the polystyrene (PS) beads coated glass, -having a 3D structure-. The 3D structured substrate was aimed to increase antibody anchorage sites. Then, the optimization of buffer media was carefully investigated with respect to non-specific protein binding. As a first step towards real sample analysis, a proof of principle of on/off cytokine detection has been analyzed in the presence of human serum. High-density one-step immobilization of anti-TNF-α antibody onto nanobead-coated glass slide chip has been demonstrated to be a promising device for application in in vitro diagnostic and profiling of cytokines with potential application toward personalized therapeutic interventions.
Starting Page	189
Ending Page	197
Page Count	9
File Format	PDF
ISSN	19760280
Journal	BioChip Journal
Volume Number	10
Issue Number	3
e-ISSN	20927843
Language	English
Publisher	The Korean BioChip Society (KBCS)
Publisher Date	2016-04-10
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunoassay Polystyrene beads Chip TNF-α Rheumatic diseases Biotechnology Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomedical Engineering Electrical and Electronic Engineering Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Non-labeled immunoassay based on zeta-potential analysis

Serological tests for the diagnosis of infectious diseases

Recent trends in the detection of pathogenic Escherichia coli O157 : H7

Optimized magnetic bead-based immunoassay for automated detection of protein toxins

A reagent-ready-on-chip microfluidic immunoassay system for rapid detection of influenza A H1N1 and H3N2 viruses

Fabrication of a hydrophobic/hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay

Antibody immobilization on to polystyrene substrate—on-chip immunoassay for horse IgG based on fluorescence

Optimization of a FACS based-immunoassay using E. coli autodisplaying Z-domains

A compact and integrated immunoassay with on-chip dispensing and magnetic particle handling

Micro and nanotechnology for early diagnosis and detection of rheumatic diseases-molecular markers

Similar Documents

Non-labeled immunoassay based on zeta-potential analysis

Serological tests for the diagnosis of infectious diseases

Recent trends in the detection of pathogenic Escherichia coli O157 : H7

Optimized magnetic bead-based immunoassay for automated detection of protein toxins

A reagent-ready-on-chip microfluidic immunoassay system for rapid detection of influenza A H1N1 and H3N2 viruses

Fabrication of a hydrophobic/hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay

Antibody immobilization on to polystyrene substrate—on-chip immunoassay for horse IgG based on fluorescence

Optimization of a FACS based-immunoassay using E. coli autodisplaying Z-domains

A compact and integrated immunoassay with on-chip dispensing and magnetic particle handling

Micro and nanotechnology for early diagnosis and detection of rheumatic diseases-molecular markers