NDLI: Brain Alpha- and Beta-Globin Expression after Intracerebral Hemorrhage

Content Provider	Springer Nature Link
Author	He, Yangdong Hua, Ya Lee, Jin Yul Liu, Wenquan Keep, Richard F. Wang, Michael M. Xi, Guohua
Copyright Year	2009
Abstract	Our recent study has demonstrated that hemoglobin (Hb) is present in cerebral neurons, and neuronal Hb is inducible after cerebral ischemia. In the present study, we examined the effects of intracerebral hemorrhage (ICH) on the messenger RNA (mRNA) levels of the α-globin (HbA) and the β-globin (HbB) components of Hb and Hb protein in the brain in vivo and in vitro. In vivo, male Sprague–Dawley rats received either a needle insertion (sham) or an infusion of autologous whole blood into the basal ganglia and were killed at different time points. In vitro, cultured rat brain cells were used for HbA, HbB, and Hb determination. Cultured neurons were exposed to 50 or 100 µM hemin for 24 h. Some neurons also were treated with deferoxamine, an iron chelator, or vehicle. Levels of HbA and HbB, Hb, and hemopexin, a transporter of heme, were measured. We found that HbA, HbB, and Hb are primarily expressed in neurons, with much lower expression in astrocytes and microglia. HbA, HbB, and Hb expression in the perihematomal zone was increased after ICH and Hb was localized in neurons and glia. Hemin increased HbA, HbB, and hemopexin mRNA levels in cultured neurons. Deferoxamine reduced hemin-induced neuronal Hb expression. ICH increased HbA and HbB expression in the brain, which may potentially serve to buffer the heme released during clot resolution.
Starting Page	48
Ending Page	56
Page Count	9
File Format	PDF
ISSN	18684483
Journal	Translational Stroke Research
Volume Number	1
Issue Number	1
e-ISSN	1868601X
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-01-06
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cerebral hemorrhage Hemin Hemoglobin Iron Deferoxamine Vascular Surgery Neurosurgery Cardiology Neurology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cardiology and Cardiovascular Medicine Neuroscience Neurology (clinical)

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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