NDLI: Waste Bread as a Biomass Source: Optimization of Enzymatic Hydrolysis and Relation between Rheological Behavior and Glucose Yield

Content Provider	Springer Nature Link
Author	Sükrü Demirci, A. Palabıyık, Ibrahim Gümüs, Tuncay Özalp, Şeymanur
Copyright Year	2016
Abstract	Amongst the many forms of food waste, bread is a major contributor to the problem. Two aims of this research investigate waste breads potential of being a bioresource for the production of fermentable sugars which are precursor of valuable bioproducts by fermentation process: finding optimum substrate, water and enzyme ratio to produce the highest amount of fermentable sugars and investigating the rheological behavior of the system during hydrolysis. Two stage waste bread hydrolysis was performed with enzymes α-amylase and amyloglucosiades and response surface methodology was used to optimize substrate, water and enzyme ratio. Discovery Hybrid Rheometer-2 (TA Instruments) fitted with a parallel-plate geometry was used to investigate steady flow viscosity of the slurry during hydrolysis. 99 % of theoretical maximum glucose yield, a main fermentable sugar, is achieved by optimizing enzymatic hydrolysis conditions of waste bread at liquefaction and saccharification stages. Just after the addition of α-amylase enzyme, substantial decrease is observed in viscosity and Casson apparent yield stress of the slurry. During saccharification stage, glucose yield increases dramatically while viscosity of the slurries is very low and does not change considerably. The results imply that utilizing high concentrations of waste bread as a feedstock to produce fermentation products offers economic benefits without causing high power consumption, excessive wear on equipment, and reduced conversion which are generally expected consequences for high-solid processing.
Starting Page	775
Ending Page	782
Page Count	8
File Format	PDF
ISSN	18772641
Journal	Waste and Biomass Valorization
Volume Number	8
Issue Number	3
e-ISSN	1877265X
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-06-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Free feedstock Glucose yield Enzyme Viscosity Engineering Environment Renewable and Green Energy Industrial Pollution Prevention Waste Management/Waste Technology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Waste Management and Disposal Environmental Engineering Renewable Energy, Sustainability and the Environment

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Waste Bread as a Biomass Source: Optimization of Enzymatic Hydrolysis and Relation between Rheological Behavior and Glucose Yield