NDLI: Effects of Chlorinated Acetates on the Glutathione Metabolism and on Glycolysis of Cultured Astrocytes

Content Provider	Springer Nature Link
Author	Schmidt, Maike M. Rohwedder, Astrid Dringen, Ralf
Copyright Year	2010
Abstract	The chlorinated acetates monochloroacetate (MCA), dichloroacetate (DCA), and trichloroacetate (TCA) are generated in water disinfection processes and are formed during metabolic detoxification of industrial solvents such as trichloroethylene. In order to test for consequences of an exposure of brain cells to the different chlorinated acetates, glutathione levels and lactate production of primary astrocyte cultures were investigated as indicators for the potential of chlorinated acetates to disturb cellular detoxification processes and glucose metabolism, respectively. Application of MCA to cultured astrocytes caused a time and concentration dependent deprivation of cellular glutathione, inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity, and loss in cell viability with halfmaximal effects observed for MCA concentrations between 0.3 and 3 mM. In contrast, the presence of acetate, DCA, or TCA in a concentration of 10 mM did not compromise cell viability nor affect cellular glutathione content or GAPDH activity. However, the presence of DCA and TCA significantly lowered the rate of cellular lactate production in viable astrocytes. These data demonstrate that the extent of chlorination strongly determines the potential of chlorinated acetates to disturb glutathione and/or glucose metabolism of astrocytes.
Starting Page	628
Ending Page	637
Page Count	10
File Format	PDF
ISSN	10298428
Journal	Neurotoxicity Research
Volume Number	19
Issue Number	4
e-ISSN	14763524
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-07-14
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Monochloroacetate Dichloroacetate Trichloroacetate Glyceraldehyde-3-phosphate dehydrogenase Detoxification Neurosciences Pharmacology/Toxicology Neurobiology Cell Biology Neurochemistry Neurology
Content Type	Text
Resource Type	Article
Subject	Neuroscience Toxicology

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