NDLI: Matrix Metalloproteinases 2 and 9 Fail to Influence Drug-Induced Neuroapoptosis in Developing Rat Brain

Content Provider	Springer Nature Link
Author	Uckermann, Ortrud Luksch, Hella Stefovska, Vanya Hoehna, Yvonne Marzahn, Jenny Theil, Marlen Pesic, Mila Górkiewicz, Tomasz Gawlak, Maciej Wilczynski, Grzegorz M. Kaczmarek, Leszek Ikonomidou, Chrysanthy
Copyright Year	2010
Abstract	Matrix metalloproteinases (MMPs) play an essential role in tissue repair, cell death, and morphogenesis. The aim of the present study was to investigate potential involvement of selected MMPs in the pathogenesis of neuronal apoptosis induced by the NMDA antagonist MK-801 (dizocilpine) or the GABA$_{A}$ agonist phenobarbital in infant rats, transgenic rats overexpressing MMP-9 and MMP-9 knockout mice. Seven-day-old rats or knockout mice received intraperitoneal injections of MK-801, 1 mg/kg, or phenobarbital, 50 mg/kg. At different survival intervals following administration of the compounds (1–72 h), pups were sacrificed, tissue from different brain regions was isolated, and the expression and activity of MMP-2 and MMP-9 were analyzed by real-time PCR, western blot, and zymography. In addition, brains were fixed and processed for TUNEL staining. In all the brain regions analyzed, we found an increased number of TUNEL-positive cells 24 h after administration of MK-801. After treatment, we detected no significant increase in MMP-2 or MMP-9 mRNA expression in cortical areas. No changes in the MMP-9 protein expression or gelatinolytic activity of MMP-2 were observed in conjunction with MK-801 or phenobarbital-induced neuroapoptosis in any brain region analyzed. The extent of neurodegeneration induced by MK-801 or phenobarbital was not altered in MMP-9 transgenic rats and was increased in MMP-9 knockout mice compared to wild-type rats and mice. Treatment with the panmetalloproteinase inhibitor GM6001 did not confer protection against MK-801-induced apoptotic cell death in the developing rat brain. Our results suggest that activation of MMP-9 and MMP-2 does not contribute to pathogenesis of neuronal apoptosis caused by NMDA antagonists or GABA$_{A}$ agonists in the developing rat and mouse brain.
Starting Page	638
Ending Page	648
Page Count	11
File Format	PDF
ISSN	10298428
Journal	Neurotoxicity Research
Volume Number	19
Issue Number	4
e-ISSN	14763524
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-07-27
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Apoptosis Development Antiepileptic Sedative Neurosciences Pharmacology/Toxicology Neurobiology Cell Biology Neurochemistry Neurology
Content Type	Text
Resource Type	Article
Subject	Neuroscience Toxicology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Sevoflurane-Induced Endoplasmic Reticulum Stress Contributes to Neuroapoptosis and BACE-1 Expression in the Developing Brain: The Role of eIF2α

Caffeine Protects Against Anticonvulsant-Induced Neurotoxicity in the Developing Rat Brain

Lipoteichoic Acid Induces Matrix Metalloproteinase-9 Expression via Transactivation of PDGF Receptors and NF-κB Activation in Rat Brain Astrocytes

Oxidized Low-Density Lipoprotein-Induced Matrix Metalloproteinase-9 Expression via PKC-δ/p42/p44 MAPK/Elk-1 Cascade in Brain Astrocytes

Ouabain-Induced Cochlear Degeneration in Rat

Zinc and the ERK Kinases in the Developing Brain

Poster abstracts ( Neurotoxicity Research , Volume 9 , Issue 2-3 )

Cannabinoids and gene expression during brain development

Comparative Neuroprotective Effects of Rasagiline and Aminoindan with Selegiline on Dexamethasone-Induced Brain Cell Apoptosis

Matrix Metalloproteinases 2 and 9 Fail to Influence Drug-Induced Neuroapoptosis in Developing Rat Brain

Similar Documents

Sevoflurane-Induced Endoplasmic Reticulum Stress Contributes to Neuroapoptosis and BACE-1 Expression in the Developing Brain: The Role of eIF2α

Caffeine Protects Against Anticonvulsant-Induced Neurotoxicity in the Developing Rat Brain

Lipoteichoic Acid Induces Matrix Metalloproteinase-9 Expression via Transactivation of PDGF Receptors and NF-κB Activation in Rat Brain Astrocytes

Oxidized Low-Density Lipoprotein-Induced Matrix Metalloproteinase-9 Expression via PKC-δ/p42/p44 MAPK/Elk-1 Cascade in Brain Astrocytes

Ouabain-Induced Cochlear Degeneration in Rat

Zinc and the ERK Kinases in the Developing Brain

Poster abstracts ( Neurotoxicity Research , Volume 9 , Issue 2-3 )

Cannabinoids and gene expression during brain development

Comparative Neuroprotective Effects of Rasagiline and Aminoindan with Selegiline on Dexamethasone-Induced Brain Cell Apoptosis

Matrix Metalloproteinases 2 and 9 Fail to Influence Drug-Induced Neuroapoptosis in Developing Rat Brain