NDLI: Chitooligosaccharides Attenuate Cu$^{2+}$-Induced Cellular Oxidative Damage and Cell Apoptosis Involving Nrf2 Activation

Content Provider	Springer Nature Link
Author	Huang, Han Chang Hong, Liang Chang, Ping Zhang, Jiao Lu, Shu Yan Zheng, Bo Wen Jiang, Zhao Feng
Copyright Year	2014
Abstract	Alzheimer’s disease (AD) is one of the common neurodegenerative diseases. Increase of labile copper pool plays an important role in the pathogenesis of AD. Nrf2(NF-E2-related factor-2)-ARE (antioxidant response element) signaling is an important intracellular manner to defend against oxidative stress. In this study, we used SH-SY5Y cells as a model of neuron to test the effect of chitooligosaccharides (COSs) on Cu$^{2+}$-induced oxidative damage. SH-SY5Y cells were treated with different concentrations of COSs (100–800 mg/L) before incubated with Cu$^{2+}$. Cell viability and cell damage and apoptosis were assessed. Both extracellular H$_{2}$O$_{2}$ and intracellular ROS were measured and the relative levels of Nrf2, phosphorylated Nrf2, and HO-1 were analyzed by Western blotting, and further HO-1 mRNA was relatively quantified by real-time quantitative PCR. The results indicated that Cu$^{2+}$-induced decrease of cell viability and increase of LDH release. In cell-free solution, COSs alone or with Cu$^{2+}$ cannot scavenge O$_{2}$ $^{−}$; however, COSs downregulate the levels of cellular oxidative stress and activated Caspase-3 induced by Cu$^{2+}$. Further, the levels of pSer40-Nrf2 protein and both the transcription and the translation of HO-1 gene are dramatically increased in COSs-protective group compared with Cu$^{2+}$ damage group. Therefore, these results indicate that Nrf2 activation might be involved in the protection of COSs against Cu$^{2+}$-induced cellular oxidative damage. COSs contribute to the attenuation of oxidative damage and could be used as a nutritional agent for AD treatment.
Starting Page	411
Ending Page	420
Page Count	10
File Format	PDF
ISSN	10298428
Journal	Neurotoxicity Research
Volume Number	27
Issue Number	4
e-ISSN	14763524
Language	English
Publisher	Springer US
Publisher Date	2014-12-27
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Alzheimer’s disease Chitooligosaccharides Cu$^{2+}$ Nrf2 Oxidative damage Neurosciences Neurology Neurochemistry Pharmacology/Toxicology Neurobiology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Neuroscience Toxicology

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