NDLI: Expression of HSP70 in response to heat-shock and its cDNA cloning from Mediterranean blue mussel

Content Provider	Springer Nature Link
Author	Toyohara, Haruhiko Hosoi, Masatomi Hayashi, Isao Kubota, Satoshi Hashimoto, Hisashi Yokoyama, Yoshihiro
Copyright Year	2005
Abstract	Expression of HSP70 in response to heat-shock was investigated at the protein and mRNA levels in Mediterranean blue mussel. Western and Northern blot analyses revealed that HSP70 was expressed following heat-shock in the mantle at both protein and mRNA levels, suggesting that gene expression of HSP70 is implicated in the cellular response to heat-shock stress in mussel. It was then attempted to clone HSP70 cDNA in order to determine the primary structure of mussel HSP70. As a result, two full-length cDNA encoding HSP70 were isolated from a cDNA library prepared from the heat-shocked mantle. The isolated cDNA consist of single open reading frames of 2067 bp and 1911 bp which encode proteins of 689 amino acids and 637 amino acids, respectively. Both HSP70 cDNA encode an ATPase do main, and a substrate-binding do main in addition to a Glu-Glu-Val-Asp (EEVD) peptide motif that is specific for cytosolic HSP70. These findings suggest that the cDNA clones obtained in the present study encode cytosolic HSP70.
Starting Page	327
Ending Page	332
Page Count	6
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	71
Issue Number	2
e-ISSN	14442906
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-01-01
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	heat-shock HSP70 mantle Mediterranean blue mussel Mytilus galloprovin-cialis stress Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Expression of taurine transporter in response to hypo-osmotic stress in the mantle of Mediterranean blue mussel

CDNA cloning and expression analysis of two heat shock protein genes, Hsp90 and Hsp60, from a sterile Ulva pertusa (Ulvales, Chlorophyta)

Identification and expression analysis of two HSP70 isoforms in mandarin fish Siniperca chuatsi

Cytotoxic and antibacterial properties of Mytilus galloprovincialis, Ostrea edulis and Crassostrea gigas (bivalve molluscs) hemolymph

Effect of a dietary fermented vegetable product on the heat shock response of Japanese flounder Paralichthys olivaceus

Molecular cloning and expression of the heat shock protein 70 gene in the Kumamoto oyster Crassostrea sikamea

Cloning of a cDNA encoding the tail region of non-muscle myosin II from the mantle tissue of scallop Patinopecten yessoensis

Induced thermotolerance and expression of heat shock protein 70 in sea cucumber Apostichopus japonicus

Molecular characterization and expression analysis of heat shock proteins 40, 70 and 90 from kuruma shrimp Marsupenaeus japonicus

Expression of HSP70 in response to heat-shock and its cDNA cloning from Mediterranean blue mussel

Similar Documents

Expression of taurine transporter in response to hypo-osmotic stress in the mantle of Mediterranean blue mussel

CDNA cloning and expression analysis of two heat shock protein genes, Hsp90 and Hsp60, from a sterile Ulva pertusa (Ulvales, Chlorophyta)

Identification and expression analysis of two HSP70 isoforms in mandarin fish Siniperca chuatsi

Cytotoxic and antibacterial properties of Mytilus galloprovincialis, Ostrea edulis and Crassostrea gigas (bivalve molluscs) hemolymph

Effect of a dietary fermented vegetable product on the heat shock response of Japanese flounder Paralichthys olivaceus

Molecular cloning and expression of the heat shock protein 70 gene in the Kumamoto oyster Crassostrea sikamea

Cloning of a cDNA encoding the tail region of non-muscle myosin II from the mantle tissue of scallop Patinopecten yessoensis

Induced thermotolerance and expression of heat shock protein 70 in sea cucumber Apostichopus japonicus

Molecular characterization and expression analysis of heat shock proteins 40, 70 and 90 from kuruma shrimp Marsupenaeus japonicus

Expression of HSP70 in response to heat-shock and its cDNA cloning from Mediterranean blue mussel