NDLI: Molecular cloning and expression of the heat shock protein 70 gene in the Kumamoto oyster Crassostrea sikamea

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Fisheries Science : Volume 83, Issue 1, January 2017

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Molecular cloning and expression of the heat shock protein 70 gene in the Kumamoto oyster Crassostrea sikamea

Content Provider	Springer Nature Link
Author	Nagata, Taisei Sameshima, Mamoru Uchikawa, Taku Osafune, Natsumi Kitano, Takeshi
Copyright Year	2017
Abstract	Episodes of summer mortality of the Kumamoto oyster Crassostrea sikamea are a major problem for its cultivation. Expression of the heat shock protein 70 (HSP70) is induced by various environmental stresses, including heat. We cloned and sequenced hsp70 complementary DNA from C. sikamea to investigate the relationship between hsp70 expression and heat tolerance in this oyster. Quantitative real-time polymerase chain reaction was performed using gill tissue dissected from oysters before and after heat shock for 1 h. The results showed hsp70 expression was faster and greater in oysters cultured at 20–22 °C than at 10–12 °C, and survival was lower among oysters cultured at 20–22 °C than at 10–12 °C. Moreover, heat tolerance was investigated by a 1-h pre-heat treatment, followed by exposure to heat shock conditions 5 days later. Survival was higher and hsp70 expression was notably lower in oysters that received the pre-heat treatment compared with those that did not. We conclude that a pre-heat treatment of only 1 h may be useful for inducing heat tolerance in C. sikamea, and that a low level of hsp70 expression after heat shock is an important index in selecting for high heat tolerance in these oysters.
Starting Page	273
Ending Page	281
Page Count	9
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	83
Issue Number	2
e-ISSN	14442906
Language	English
Publisher	Springer Japan
Publisher Date	2017-01-27
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Stress response Quantitative real-time polymerase chain reaction Heat tolerance Pre-heat treatment Molecular chaperone Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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