NDLI: The development of oocyte cryopreservation techniques in blue mussels Mytilus galloprovincialis

Content Provider	Springer Nature Link
Author	Wang, Hanru Li, Xiaoxu Wang, Meiqing Clarke, Steven Gluis, Mark
Copyright Year	2014
Abstract	Reliable techniques for the cryopreservation of both sperm and oocytes of the blue mussel Mytilus galloprovincialis Lamarck would increase the availability of seed supplies out-of-season and enhance efficiency in selective breeding. We have investigated the optimal cryo-technique for blue mussel oocytes. The toxicity of three cryoprotective agents (CPAs) [dimethyl sulfoxide (DMSO), ethylene glycol (EG) and propylene glycol (PG)] at different concentrations (1–5 M) and exposure times (0.25–30 min) were investigated for mussel oocytes at room temperature (20 °C) or on ice. The same CPAs (1, 1.5 and 2 M) as well as three different cryoprotectant mixtures [1.5 M EG + 0.2 M trehalose + 100 % Milli-Q water (EGTM); 1.5 M EG + 0.2 M trehalose + 75 % Milli-Q water + 25 % seawater; 1.5 M EG + 0.2 M sucrose + 100 % Milli-Q water] were tested by comparing the post-thaw oocyte fertilization rate after using the slow-cooling method. Vitrification was also examined; however, this method failed to produce any post-thaw surviving oocytes. Among the tested CPAs, EG was the least toxic to oocytes. There was a tendency for the equilibration of CPAs on ice to achieve a higher oocyte fertilization rate compared with that at room temperature, and this difference was significant at concentrations of 3 and 4 M (P < 0.01). The DMSO, EG and PG treatments all resulted in post-thaw fertilization, with EGTM achieving the highest number of surviving oocytes (32 %). At the optimal seeding temperature (−7 °C), the addition of 0.2 M trehalose to EG resulted in a better fertilization rate of post-thawed oocytes than the addition of 0.2 M sucrose. All of the treatments evaluated produced D-larvae from post-thawed oocytes, although the rates were low.
Starting Page	1257
Ending Page	1267
Page Count	11
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	80
Issue Number	6
e-ISSN	14442906
Language	English
Publisher	Springer Japan
Publisher Date	2014-08-28
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Slow-cooling method Vitrification Cryoprotectant Oocytes Cryopreservation Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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