NDLI: Simple differentiation of two closely related species Porphyra tenera and Porphyra yezoensis (Bangiophyceae, Rhodophyta) based on length polymorphism of actin-related protein 4 gene (ARP4)

Content Provider	Springer Nature Link
Author	Park, Eun Jeong Endo, Hirotoshi Kitade, Yukihiro Saga, Naotsune
Copyright Year	2008
Abstract	Genetic polymorphisms were investigated to develop a simple and rapid method to differentiate between the two closely related species, Porphyra tenera and Porphyra yezoensis. Polymerase chain reaction (PCR) using the specific primer pair of the ARP4 gene gave length polymorphic single fragments of genomic DNAs from five strains of P. tenera (Japan T-8, JTW; Korea KTY1, KTY2, KTY3) and seven strains of P. yezoensis (Japan TU-1, TU-2, TUH-25, JHU, JA-1; Korea KGJ, KPH). All strains of P. yezoensis had introns 60 bp longer than that of P. tenera. Multiple cleaved amplified polymorphic sequence (CAPS) markers were also developed to differentiate P. tenera and P. yezoensis. This is the first report of length polymorphisms that can be used to differentiate between the how species using only PCR amplification with agarose gel electrophoresis. It is expected that the length polymorphism and plentiful CAPS profiles obtained in this study will be useful in the assessment of genetic diversity within P. tenera and P. yezoensis as well as in breeding science that requires the collection of various strains of the two species.
Starting Page	613
Ending Page	620
Page Count	8
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	74
Issue Number	3
e-ISSN	14442906
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-01-01
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ARP4 CAPS marker endangered species length polymorphism Porphyra tenera Porphyra yezoensis Rhodophyta SSU rDNA Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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Genetic polymorphism withinPorphyra yezoensis(Bangiales, Rhodophyta) and related species from Japan and Korea detected by cleaved amplified polymorphic sequence analysis

Identification of currently cultivated Porphyra species by PCR-RFLP analysis

Characterization of a cDNA encoding a homolog of actin-related protein 4 from a marine red alga Porphyra yezoensis

DNA content of the cell nucleus in the macroalga Porphyra yezoensis (Rhodophyta)

A new primer for 16S rDNA analysis of microbial communities associated with Porphyra yezoensis

Inheritance pattern of chloroplast and mitochondrial genomes in artificial hybrids of Porphyra yezoensis (Rhodophyta)

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Molecular characterization and expression analysis of two Na$^{+}$/H$^{+}$ antiporter genes in the marine red alga Porphyra yezoensis

Simple differentiation of two closely related species Porphyra tenera and Porphyra yezoensis (Bangiophyceae, Rhodophyta) based on length polymorphism of actin-related protein 4 gene (ARP4)

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