NDLI: Species identification method for Scombrops boops and Scombrops gilberti based on polymerase chain reaction-restriction fragment length polymorphism analysis of mitochondrial DNA

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Fisheries Science : Volume 74, Issue 2, March 2008

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Fisheries Science : Volume 73

Fisheries Science : Volume 72

Fisheries Science : Volume 71

Species identification method for Scombrops boops and Scombrops gilberti based on polymerase chain reaction-restriction fragment length polymorphism analysis of mitochondrial DNA

Content Provider	Springer Nature Link
Author	Itoi, Shiro Takai, Noriyuki Naya, Satomi Dairiki, Keitaro Yamada, Akira Akimoto, Seiji Yoshihara, Kiyoshi Sugita, Haruo
Copyright Year	2008
Abstract	Gnomefish Scombrops boops and Scombrops gilberti are commercially important fishes in Japan, but these species are often confused in the markets because of their morphological similarity. To identify these two species, we performed nucleotide sequencing and restriction fragment length polymorphism (RFLP) analysis on 16S ribosomal RNA (rRNA) gene and the control region in mitochondrial DNA. Five and 12 nucleotide substitutions were observed between species in the 777-bp 16S rRNA gene and 471-bp control region, respectively. Diagnostic restriction sites for discriminating between S. boops and S. gilberti were found in the 16S rRNA gene, but not in the control region. Polymerase chain reaction (PCR)-RFLP analysis using two enzymes, EcoNI and Mval, clearly discriminated between S. boops and S. gilberti identified by meristic characters. The PCR-RFLP analysis identified most of the 168 Scombrops young caught in the coastal waters of the Izu and Miura peninsulas as S. boops, suggesting that S. gilberti juveniles are rare in this area.
Starting Page	503
Ending Page	510
Page Count	8
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	74
Issue Number	3
e-ISSN	14442906
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-01-01
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	16S rRNA control region mtDNA PCR-RFLP Scombropidae Scombrops boops Scombrops gilberti Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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