NDLI: CDNA cloning and expression of a novel group IB phospholipase A$_{2}$ in the intestine of the red sea bream, Pagrus (Chrysophrys) major

Content Provider	Springer Nature Link
Author	Fujikawa, Yukichi Uematsu, Kazumasa Iijima, Noriaki
Copyright Year	2009
Abstract	A cDNA encoding a novel phospholipase A$_{2}$ (PLA$_{2}$), which was named IN PLA$_{2}$, was cloned from the intestine of the red sea bream. The amino acid sequence of IN PLA$_{2}$ showed 49–75% homology with those of red sea bream group IB sPLA$_{2}$, hepatopancreas DE-1 and DE-2 PLA$_{2}$, and gill G-3 PLA$_{2}$. IN PLA$_{2}$ consists of a prepropeptide of 24 amino acid residues, followed by a mature protein. IN PLA$_{2}$ contains 14 cysteines, and includes Cys11, the calcium binding loop and the pancreatic loop that are commonly conserved in group IB sPLA$_{2}$ enzymes. In addition, IN PLA$_{2}$ is a cationic protein with a pI of 8.52. Therefore, IN PLA$_{2}$ was identified as a novel group IB sPLA$_{2}$ isoform in red sea bream. IN PLA$_{2}$ mRNA was found by northern blot analysis to be expressed mainly in the pyloric caeca and the intestine, and was detected in the goblet cells of the intestine by in situ hybridization. The expression level of IN PLA$_{2}$ mRNA was elevated by intravenous injection of lipopolysaccharide—the outer-membrane component of Gram-negative bacteria. These results suggest that IN PLA$_{2}$ is secreted from the goblet cells of the intestine in response to stimulus such as bacterial infection, and that it contributes to antimicrobial defense in addition to the digestion of dietary phospholipids in the gastrointestinal tract.
Starting Page	453
Ending Page	461
Page Count	9
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	75
Issue Number	2
e-ISSN	14442906
Language	English
Publisher	Springer Japan
Publisher Date	2009-02-07
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Goblet cells Intestine Phospholipase A$_{2}$ Red sea bream Secretory Lipopolysaccharide Food Science Freshwater & Marine Ecology Fish & Wildlife Biology & Management
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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