NDLI: Characterization of gelatinolytic enzymes in the skeletal muscle of red sea bream Pagrus major

Content Provider	Springer Nature Link
Author	Yoshida, Asami Bae, Inwoo Sonoda, Hiroko Masuo, Ryuichi Oda, Sachie Cao, Min Jie Osatomi, Kiyoshi Hara, Kenji
Copyright Year	2009
Abstract	Gelatinolytic enzymes were partially purified from the skeletal muscle of red sea bream Pagrus major and characterized to obtain information on post mortem tenderization of fish muscle. Four gelatinolytic activities, G1 (90 kDa), G2 (65 kDa), G3 (60 kDa), and G4 (100 kDa), were detected in the Q Sepharose column. G1, the major gelatinolytic enzyme, and G4 were identified as serine proteinases from results of inhibitor spectrum and substrate specificity. By contrast, G2 and G3 were found to be metalloproteinases since these were inhibited by ethylenediamine tetraacetic acid and o-phenanthroline, and activated by 4-aminophenylmercuric acetate. The optimum pH and temperature of these enzymes were in the ranges of 7–9 and 20–40°C, respectively.
Starting Page	1317
Ending Page	1322
Page Count	6
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	75
Issue Number	5
e-ISSN	14442906
Language	English
Publisher	Springer Japan
Publisher Date	2009-07-29
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gelatinolytic enzyme Metalloproteinase Muscle Red sea bream Serine proteinase Food Science Freshwater & Marine Ecology Fish & Wildlife Biology & Management
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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Characterization of gelatinolytic enzymes in the skeletal muscle of red sea bream Pagrus major