NDLI: Proteomics Analysis of Embryo and Endosperm from Mature Common Buckwheat Seeds

Content Provider	Springer Nature Link
Author	Kamal, Abu Hena Mostafa Jang, Ill Dong Kim, Da Eun Suzuki, Tatsuro Chung, Keun Yook Choi, Jong Soon Lee, Moon Soon Park, Cheol Ho Park, Sang Un Lee, Seong Hee Jeong, Heon Sang Woo, Sun Hee
Copyright Year	2011
Abstract	We used proteomics analysis to generate the profiles of proteins in the endosperm and embryo of common buckwheat grains. These differentially expressed proteins are potentially involved in seed metabolism. Extractions were done by trichloroacetic acid (TCA) precipitation. The resulting proteins were separated using SDS-PAGE coupled to LC-ESI-Q/TOF-MS/MS. This allowed us to detect and identify 67 proteins with isoforms, making this the most inclusive protein profile. The proteins were determined to be functionally involved in the central metabolic pathway of the seed, with metabolic interest being reflected in the occurrence of a tissue-specific enzyme balance. For a case in point, we found a tissue-specific and subcellular compartment-specific isoform of granule-bound starch synthase 1 in the chloroplast/amyloplast. This provided proteomic verification of the presence of a distinct regulatory mechanism for the biosynthesis of glycan and starch, which produce amylase and amylopectin. Furthermore, several previously characterized allergenic proteins such as 11S and 13S globulin seed storage protein were acknowledged in our seed samples, thus representing the potential for proteomics techniques that survey food sources for any incidence of allergens. This protein profile of common buckwheat grain is a new avenue for understanding its seed physiology in dormant stage as well as suggesting commercial applications for the buckwheat industry as buckwheat flour.
Starting Page	81
Ending Page	91
Page Count	11
File Format	PDF
ISSN	12269239
Journal	Journal of Plant Biology
Volume Number	54
Issue Number	2
e-ISSN	18670725
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-02-11
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Common buckwheat Embryo Endosperm Proteomics Plant Systematics/Taxonomy/Biogeography Plant Ecology Plant Breeding/Biotechnology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Proteomic analysis of the rice endosperm starch-deficient mutants osagps2 and osagpl2

Analysis of the Cold-Responsive Transcriptome in the Mature Pollen of Arabidopsis

Morphology and anatomy of mature embryos and seedlings in parasitic angiospermCuscuta japonica

Genome-wide identification and extensive analysis of rice-endosperm preferred genes using reference expression database

Isolation and characterization of the C-class MADS-box gene from the distylous pseudo-cereal Fagopyrum esculentum

Biotic Stress-Responsive Rice Proteome: An Overview

FaesPI, a Fagopyrum esculentum PISTILLATA ortholog, is involved only in stamen development

Analysis of gradient-like expression of miR167 in Arabidopsis thaliana embryonic tissue

Molecular cytogenetics of plant genome

Proteomics Analysis of Embryo and Endosperm from Mature Common Buckwheat Seeds

Similar Documents

Proteomic analysis of the rice endosperm starch-deficient mutants osagps2 and osagpl2

Analysis of the Cold-Responsive Transcriptome in the Mature Pollen of Arabidopsis

Morphology and anatomy of mature embryos and seedlings in parasitic angiospermCuscuta japonica

Genome-wide identification and extensive analysis of rice-endosperm preferred genes using reference expression database

Isolation and characterization of the C-class MADS-box gene from the distylous pseudo-cereal Fagopyrum esculentum

Biotic Stress-Responsive Rice Proteome: An Overview

FaesPI, a Fagopyrum esculentum PISTILLATA ortholog, is involved only in stamen development

Analysis of gradient-like expression of miR167 in Arabidopsis thaliana embryonic tissue

Molecular cytogenetics of plant genome

Proteomics Analysis of Embryo and Endosperm from Mature Common Buckwheat Seeds