NDLI: Isolation, purification, characterization of invertase inhibitor from sugarbeet roots and its role in the sucrose inversion

Content Provider	Springer Nature Link
Author	Jaidka, Aarti Mann, A. P. S. Sawhney, Sukhminder
Copyright Year	1999
Abstract	The invertase inhibitor was isolated, purified and characterized biochemically and electrophorectically from sugarbeet roots. Acid and alkaline invertase was found in roots of sugarbeet. The acid invertase activity decreased during development whereas the alkaline invertase persisted throughout the foot development. The decreased activity of the acid invertase can be attributed to the presence of high inhibitor concentration at the maturity level. To characterize, the invertase inhibitor was isolated from the roots of H1-0064 variety of sugarbeet and purified to 104.5 folds using Sephadex G-100 column chromatography. Non-denaturing polyacrylamide gel electrophoresis of the ammonium sulphate and acetone preparation of the inhibitor showed S and 3 bands respectively, whereas the purified invertase inhibitor revealed single protein band near the dye-front, confirming the homogeneity and low molecular weight of the sugarbeet inhibitor. The inhibitor tested against theCandida utilis invertase expressed optimum activity at pH 4.0 and 40°C. The inhibitor binds the enzyme in a reversible manner and was found to exhibit non-competitive mode of action representing constant K$_{m}$ with gradual decrease in V$_{max}$ of the enzyme.
Starting Page	63
Ending Page	68
Page Count	6
File Format	PDF
ISSN	09721525
Journal	Sugar Tech
Volume Number	6
Issue Number	1-2
e-ISSN	09740740
Language	English
Publisher	Springer India
Publisher Date	1999-01-01
Publisher Place	New Delhi
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sugarbeet purification characterization and PAGE Agriculture
Content Type	Text
Resource Type	Article
Subject	Agronomy and Crop Science

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