NDLI: In vitro propagation and withaferin A production in Withania ashwagandha, a rare medicinal plant of India

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In vitro propagation and withaferin A production in Withania ashwagandha, a rare medicinal plant of India

Content Provider	Springer Nature Link
Author	Mir, Bilal Ahmad Mir, Shabir Ahmad Koul, Sushma
Copyright Year	2014
Abstract	Withania ashwagandha, belonging to the family Solanaceae, is an important medicinal herb of India with restricted geographic distribution. It is a rich source of withaferin A (WA) and other bioactive withanolides. In the present study a rapid in vitro mass propagation protocol of W. ashwagandha was developed from nodal explants. Nodal explants were cultured on MS medium supplemented with various concentrations and combinations of plant growth regulators (PGRs). The highest number of regenerated shoots per ex-plant (33 ± 2.7) and highest WA (13.4 ± 1.15 mg/g of DW) production was obtained on MS medium supplemented with 5.0 μM 6-benzyladenine (BA) and 1.0 μM Kinetin (Kn). In vitro raised shoots were further rooted on half-strength MS medium containing 2.0 μM Indole-3-butyric acid (IBA) and analyzed for WA production. The rooted plantlets when transferred to poly bags in the greenhouse showed 90 % survival frequency. Levels of WA were higher in the in vitro and ex vitro derived shoot and root tissues as compared to field grown mother plants. In an attempt to further maximize WA production, shoot cultures were further grown in liquid MS medium supplemented with 5.0 μM 6-benzyladenine (BA) and 1.0 μM Kinetin (Kn). Root cultures were grown on half strength MS liquid medium fortified with 2.0 μM of IBA. WA production in the liquid cultures was significantly higher compared to the static composition of the same media. This protocol, first of its kind in this plant, can be successfully employed for conservation, proliferation and large-scale production of WA. The regenerated plants can also be used in traditional medicine as an alternative to naturally collected plants.
Starting Page	357
Ending Page	364
Page Count	8
File Format	PDF
ISSN	09715894
Journal	Physiology and Molecular Biology of Plants
Volume Number	20
Issue Number	3
e-ISSN	09740430
Language	English
Publisher	Springer India
Publisher Date	2014-06-12
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	High performance liquid chromatography In vitro conservation Medicinal plant Plant growth regulators W. ashwagandha Withaferin A Plant Sciences Plant Physiology Biophysics and Biological Physics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Molecular Biology

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