NDLI: Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal

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Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal

Content Provider	Springer Nature Link
Author	Chakraborty, Nilanjan Banerjee, Debarupa Ghosh, Moumita Pradhan, Prakash Gupta, Namrata Shanu Acharya, Krishnendu Banerjee, Maitreyi
Copyright Year	2012
Abstract	Withania somnifera (L.) Dunal, is an important medicinal plant being the source of extremely important compounds like withanolides and withaferin. Influence of different plant growth regulators (PGRs) were evaluated for induction of callus, callus mediated regeneration and production of secondary metabolites in them. Explants for callusing were collected from plants grown in vitro and maximum callusing (98 %) was obtained on MS medium supplemented with a combination of 2,4-dichlorophenoxy acetic acid (2,4-D) (0.5 mg l$^{-1}$) and kinetin (KN) (0.2 mg l$^{−1}$). Among different types of calli, best shoot regeneration was observed on green, compact calli produced on MS medium with a combination of 6-benzylamino purine (BAP) and indole butyric acid (IBA). MS medium supplemented with BAP (2 mg l$^{−1}$) showed highest frequency (98 %) of shoot bud regeneration. The micro-shoots were efficiently rooted on MS media supplemented with 0.5 mg l$^{−1}$ IBA. Rooted plants were transferred to soil-vermi-compost (1:3; w/w) medium in greenhouse for acclimatization. Presence of withanolide A and withaferin A in calli was validated through high performance thin layer chromatography (HPTLC). It was interesting to observe that the PGRs showed significant influence on the secondary metabolites production in callus and 2,4-D having the least effect. Histological studies revealed the origin of shoot tip in the callus during regeneration.
Starting Page	117
Ending Page	125
Page Count	9
File Format	PDF
ISSN	09715894
Journal	Physiology and Molecular Biology of Plants
Volume Number	19
Issue Number	1
e-ISSN	09740430
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-10-30
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Callusing High performance thin layer chromatography Histology Plant growth regulator Shoot bud regeneration Withaferin A Withanolide A Plant Sciences Plant Physiology Biophysics and Biological Physics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Molecular Biology

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Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal

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Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal

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Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal