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| Content Provider | Springer Nature Link |
|---|---|
| Author | Lee, Heetae Park, YoungBin Kim, Misoon Jee, Youngmee Cheon, Doo sung Jeong, Hae Sook Ko, GwangPyo |
| Copyright Year | 2010 |
| Abstract | Norovirus (NoV) is the leading cause of acute gastroenteritis worldwide. Currently, reverse transcription polymerase chain reaction (RT-PCR) is used commonly to detect NoVs in both clinical and environmental samples. However, RT-PCR requires expensive equipment and cannot be performed on site. In this study, a latex agglutination test (LAT) using antibody-labeled latex beads for detecting NoVs was developed. Two kinds of polyclonal antibodies, one generated from synthetic peptides and the other from E. coli-expressed NoV capsid proteins, were used to develop the LAT. Each of these polyclonal antibodies was immobilized on the surface of latex beads and tested for the ability to detect NoVs. Under optimized conditions, our LAT detected GII.4 NoV at concentrations as low as 3.3×10$^{5}$ RT-PCR units/ml in stool samples. The detection limit for the LAT was approximately 1.7 103 RT-PCR units. Forty-eight stool samples were tested for NoVs using this LAT. In comparison with an RT-PCR assay, the sensitivity and specificity of the LAT were 35% and 100%, respectively. With further optimization, this LAT used with appropriate antibodies could be applied for convenient detection of NoVs in clinical diagnosis and food monitoring. |
| Starting Page | 419 |
| Ending Page | 425 |
| Page Count | 7 |
| File Format | |
| ISSN | 12258873 |
| Journal | Journal of Microbiology |
| Volume Number | 48 |
| Issue Number | 4 |
| e-ISSN | 19763794 |
| Language | English |
| Publisher | The Microbiological Society of Korea |
| Publisher Date | 2010-08-20 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | polyclonal antibody latex agglutination test (LAT) norovirus rapid detection sensitivity specificity Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Applied Microbiology and Biotechnology |
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