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| Content Provider | Springer Nature Link |
|---|---|
| Author | Jitprasutwit, Siroj Thaewpia, Wisansanee Muangsombut, Veerachat Lulitand, Aroonlug Leelayuwat, Chanvit Lertmemongkolchai, Ganjana Korbsrisate, Sunee |
| Copyright Year | 2010 |
| Abstract | Burkholderia thailandensis is a close relative of Burkholderia pseudomallei. These organisms are very similar, but B. thailandensis is far less virulent than B. pseudomallei. Nucleotide sequencing and analysis of 14 B. thailandensis isolates revealed variation in the regions coding for the type III secreted BipD protein. The degree of B. thailandensis BipD sequence variation was greater than that found in B. pseudomallei. Western blot analysis indicated that, unlike B. pseudomallei, B. thailandensis type III secreted proteins including BipD and BopE could not be detected in the supernatant of culture medium unless induced by acidic conditions. In addition, culturing B. thailandensis under acidic growth conditions (pH 4.5) can induce the ability of this bacterium to invade human respiratory epithelial cells A549. The identification of an environmental stimulus that increases the invasion capability of B. thailandensis invasion is of value for those who would like to use this bacterium as a model to study B. pseudomallei virulence. |
| Starting Page | 526 |
| Ending Page | 532 |
| Page Count | 7 |
| File Format | |
| ISSN | 12258873 |
| Journal | Journal of Microbiology |
| Volume Number | 48 |
| Issue Number | 4 |
| e-ISSN | 19763794 |
| Language | English |
| Publisher | The Microbiological Society of Korea |
| Publisher Date | 2010-08-20 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | B. thailandensis acidic pH type III secretion system invasion Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Applied Microbiology and Biotechnology |
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